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Histotechnology HTL Exam - Comprehensive Review Questions and Answers (Latest Update 2025)
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Histotechnology HTL Exam - Comprehensive Review Questions and Answers (Latest Update 2025)
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Histotechnology HTL Exam - ComprehensiveReview Questions and Answers (Latest
Update 2025)
Alcian Blue demonstrates - Correct Answer ✅Acid mucins
Perl's Prussian Blue shows - Correct Answer ✅Hemosiderin
Southgate's mucicarmine - Correct Answer ✅epithelial acid
mucins
Verhoeff's Van Gieson - Correct Answer ✅elastic
Van Kossa shows - Correct Answer ✅calcium
Ziehl Neelsen shows - Correct Answer ✅Acid fast bacilli
(T.B.)
Nuclei in Gomori's Aldehyde Fuchsin technique - Correct
Answer ✅yellow - no specific stain for nuclei
Mast cells in a Gomori's Aldehyde Fuchsin technique -
Correct Answer ✅purple - GAF not specific for elastic
Gram positive bacteria if over-differentiated after crystal
violet - Correct Answer ✅red
,Histotechnology HTL Exam - Comprehensive
Review Questions and Answers (Latest
Update 2025)
Nuclei in the Brown and Brenn Gram stain if over
differentiated with picric acid/acetone - Correct Answer
✅yellow
Fungi in the Periodic Acid Schiff (PAS) technique - Correct
Answer ✅magenta - chitin binds with Schiffs
Fungi in the Grocott's Methenamine Silver technique -
Correct Answer ✅black - silver impregnation
Neutral mucins in a PAS technique if potassium
permanganate is used as the oxidizing agent - Correct
Answer ✅light pink - colour of background - CHO
overoxidized from aldehydes to acid state
Cytoplasm in an H&E if over differentiated with acid alcohol -
Correct Answer ✅pink - only affects nuclei
Epithelial acid mucins in the Southgate's mucicarmine
technique - Correct Answer ✅red
Cytoplasm in an H&E if the blueing agent is not properly
removed - Correct Answer ✅very pale pink
,Histotechnology HTL Exam - Comprehensive
Review Questions and Answers (Latest
Update 2025)
Nuclei in the Congo Red technique - Correct Answer ✅blue
- stained with aluminum hematoxylin
Calcium in the Von Kossa technique - Correct Answer
✅black - silver impregnation
Glycogen, after diastase in the Periodic Acid Schiff technique -
Correct Answer ✅pale pink - background colour
Mycobacterium after Ziehl Neelsen technique - Correct
Answer ✅red - AFB stain with carbol fuchsin
Mycobacterium after Ziehl Neelsen technique - Correct
Answer ✅red - AFB stain with carbol fuchsin
Streptococcus after Ziehl Neelsen technique - Correct
Answer ✅blue - colour of background
Neutral lipids for Oil Red O cut on a cryostat - Correct
Answer ✅red to orange
Neutral lipids for Oil Red O cut from wax block - Correct
Answer ✅not demonstrated - removed
, Histotechnology HTL Exam - Comprehensive
Review Questions and Answers (Latest
Update 2025)
Hemosiderin after a Perl's Prussian Blue - Correct Answer
✅blue
Nuclei after Masson's trichrome using an aluminum
Hematoxylin - Correct Answer ✅red - same as cytoplasm
Fine elastic fibres in Verhoeff's Van Gieson (VVG) after leaving
in Van Gieson reagent too long - Correct Answer ✅not
black/not demonstrated - iron hematoxylin removed by the
acid
Cytoplasm if underdifferentiated in the VVG technique -
Correct Answer ✅grey to black
Reticulin fibres if iron alum is skipped in the Gordon and
Sweet's - Correct Answer ✅not demonstrated
Melanin if potassium permanganate was used as an oxidizing
agent in the Gomori Burtner technique - Correct Answer
✅not demonstrated - removed
Melanin in the Luxol fast blue if overdifferentiated with
lithium carbonate - Correct Answer ✅pink
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