BIOL 4100 Exam 3 Questions With Complete Solutions
-always has GTP bound to it, because the GTP cannot be
hydrolyzed
-added to + end of microtubule
-forms heterodimer Correct Answers alpha tubulin
-can bind GTP and hydrolyze it to form bound GDP
-forms heterodimer
-added to - end of microtubule Correct Answers beta tubulin
-class 1 type 2 MAP proteins
-bind longitudinally to microtubule
-bind to everything but neuronal tissues Correct Answers
MAP4
-class 1 type 2 MAP proteins
-if hyperphosporylated, leads to protein dissociation from
microtubule, destabilization of the microtubule and development
of paired helical filaments
-microtubule bending protein (wraps around protofilaments like
a bungee cord) Correct Answers tau proteins
-Class 1 Type 2 MAP proteins
-present in most neuronal tissues
-bind longitudinally to microtubule Correct Answers MAP2
-coexistence of growing and shrinking microtubules in some
conditions Correct Answers dynamic instability
-dark band in middle of I band
,-holds 2 microfilaments together) Correct Answers z line
-darker image
-includes H zone and m line Correct Answers a (anesotropic)
band
-hydrophilic interactions
-homodimer interactions (beta-beta and alpha-alpha)
-once reaches critical length, protofilaments interact laterally
Correct Answers inter-PF contacts
-hydrophobic interactions of about 24 subunits or 12 tubulin
dimers
-heterodimer interactions (beta and alpha) Correct Answers
intra-PF contacts
-intermediate filaments are thought to play a _____-_________
role in the cell
-in neurons, IFs are ________ transported and remodeled.
-IFs are _____ susceptible to chemical attack than are MTs and
microfilaments/ why? Correct Answers tension-bearing,
dynamically, less, more sturdy (SDS can't break it down)
-intricate network of IF structures extending from the cell
membrane to the nucleus that regulates cell shape, motility, and
organelle positioning
-ex. desmin, GFAP, peripherin, vimentin Correct Answers
class 3 IF
-lighter image
-includes z line Correct Answers i (isotropic) band
,-movement of a cell or organism through the environment
-movement of the environment past or through a cell
-movement of components in the cell Correct Answers
motility
-name the dynein structure
functionally equivalent to the lever arm (power stroke) of
mysoin--think old western revolver pistol; involved in the motor
activity of the motor/movement Correct Answers linker
domain
-name the dynein structure:
-six ATPase Associate Activity domains arranged in a clockwise
orientation containing all four nucleotide binding domains of
dynein
-ATP hydrolysis at position 6/1
-think ATP binding position is a backup (can give ATP to
piosition 6/1 if needed)
-think position 3/4 gives confornation to ring, ATP hydrolysis
doesn't happen here Correct Answers AAA+ ring domain
-stiffest and most complex assembly/disassembly
-no helper proteins
-spontaneous Correct Answers tubulin
3 actins together begins what process? Correct Answers
nucleative process (bind to different nucleotides based on
structure
, a complex network of interlinking filaments and tubules that
extend throughout the cytoplasm of a cell Correct Answers
cytoskeleton
a dimer of Class 1 or 2 intermediate filament alpha and/or beta
keratin is always a homo/hetero dimer? Correct Answers
hetero
a sarcomere is made of what. how are they measured? Correct
Answers A band (H zone and M line), I band (z line); z line to
z line
a think contractile actin shell containing myosin in its network,
underlying the inner face of the plasma membrane Correct
Answers cell cortex
act as active springs for the contractile unit, pulls microfilaments
past each other, allows cells to stretch/thin and have progressive
movement Correct Answers myosin motors
actin assembly and disassembly is an ongoing, ______ process
that builds cytoskeletal structures in the cell and also ______
actin associating proteins to constantly rebuild and remodel the
actin cytoskeleton Correct Answers dynamic, reuses
actin filaments connected by crosslinker proteins to form
networks are/are not nucleated by Arp2/3 Correct Answers are
not
-always has GTP bound to it, because the GTP cannot be
hydrolyzed
-added to + end of microtubule
-forms heterodimer Correct Answers alpha tubulin
-can bind GTP and hydrolyze it to form bound GDP
-forms heterodimer
-added to - end of microtubule Correct Answers beta tubulin
-class 1 type 2 MAP proteins
-bind longitudinally to microtubule
-bind to everything but neuronal tissues Correct Answers
MAP4
-class 1 type 2 MAP proteins
-if hyperphosporylated, leads to protein dissociation from
microtubule, destabilization of the microtubule and development
of paired helical filaments
-microtubule bending protein (wraps around protofilaments like
a bungee cord) Correct Answers tau proteins
-Class 1 Type 2 MAP proteins
-present in most neuronal tissues
-bind longitudinally to microtubule Correct Answers MAP2
-coexistence of growing and shrinking microtubules in some
conditions Correct Answers dynamic instability
-dark band in middle of I band
,-holds 2 microfilaments together) Correct Answers z line
-darker image
-includes H zone and m line Correct Answers a (anesotropic)
band
-hydrophilic interactions
-homodimer interactions (beta-beta and alpha-alpha)
-once reaches critical length, protofilaments interact laterally
Correct Answers inter-PF contacts
-hydrophobic interactions of about 24 subunits or 12 tubulin
dimers
-heterodimer interactions (beta and alpha) Correct Answers
intra-PF contacts
-intermediate filaments are thought to play a _____-_________
role in the cell
-in neurons, IFs are ________ transported and remodeled.
-IFs are _____ susceptible to chemical attack than are MTs and
microfilaments/ why? Correct Answers tension-bearing,
dynamically, less, more sturdy (SDS can't break it down)
-intricate network of IF structures extending from the cell
membrane to the nucleus that regulates cell shape, motility, and
organelle positioning
-ex. desmin, GFAP, peripherin, vimentin Correct Answers
class 3 IF
-lighter image
-includes z line Correct Answers i (isotropic) band
,-movement of a cell or organism through the environment
-movement of the environment past or through a cell
-movement of components in the cell Correct Answers
motility
-name the dynein structure
functionally equivalent to the lever arm (power stroke) of
mysoin--think old western revolver pistol; involved in the motor
activity of the motor/movement Correct Answers linker
domain
-name the dynein structure:
-six ATPase Associate Activity domains arranged in a clockwise
orientation containing all four nucleotide binding domains of
dynein
-ATP hydrolysis at position 6/1
-think ATP binding position is a backup (can give ATP to
piosition 6/1 if needed)
-think position 3/4 gives confornation to ring, ATP hydrolysis
doesn't happen here Correct Answers AAA+ ring domain
-stiffest and most complex assembly/disassembly
-no helper proteins
-spontaneous Correct Answers tubulin
3 actins together begins what process? Correct Answers
nucleative process (bind to different nucleotides based on
structure
, a complex network of interlinking filaments and tubules that
extend throughout the cytoplasm of a cell Correct Answers
cytoskeleton
a dimer of Class 1 or 2 intermediate filament alpha and/or beta
keratin is always a homo/hetero dimer? Correct Answers
hetero
a sarcomere is made of what. how are they measured? Correct
Answers A band (H zone and M line), I band (z line); z line to
z line
a think contractile actin shell containing myosin in its network,
underlying the inner face of the plasma membrane Correct
Answers cell cortex
act as active springs for the contractile unit, pulls microfilaments
past each other, allows cells to stretch/thin and have progressive
movement Correct Answers myosin motors
actin assembly and disassembly is an ongoing, ______ process
that builds cytoskeletal structures in the cell and also ______
actin associating proteins to constantly rebuild and remodel the
actin cytoskeleton Correct Answers dynamic, reuses
actin filaments connected by crosslinker proteins to form
networks are/are not nucleated by Arp2/3 Correct Answers are
not
