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Unit 2 Learning Aim C
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Mariya HussainGroup 3
10632164
Unit 2
7/03/2023
Unit 2C: Using Chromatographic techniques to identify components in
mixtures.
What is Chromatography?
Chromatography involves a solution being passed through a media
where the constituent elements of the mixture dissolves at different
speeds to allow the dissolved compounds to be separated from a
mixture.
Practical report for paper chromatography and TLC
Paper chromatography – allows low-molecular-mass compounds to be
separated based on their arrangement of the stationary and mobile
phase. I drew 4 points at the bottom to mark each solution for Histidine,
Glycine, tyrosine and the unknown sample. I added each of the samples
to the correct point and added solution 3 and air dried it. I then placed it
onto a uv light where I marked any spots that had appeared and circled
it. I then used a ruler to measure how far the solvent had travelled to the
solvent line and added all results on a graph. I interpreted these results
to find out what the Rf values were for each of the 4 samples. Large
amounts of samples would ruin the results.
TLC- separates non-volatile mixtures. I drew 4 points at the bottom to
mark each solution for Histidine, Glycine, tyrosine and the unknown
sample. I added each of the samples to the correct point and added
solution 3 and air dried it. I then placed it onto a uv light where I marked
any spots that had appeared and circled it. I then used a ruler to
measure how far the solvent had travelled to the solvent line and added
all results on a graph. I interpreted these results to find out what the Rf
values were for each of the 4 samples. Results that are found is difficult
to reproduce.
, Stationary phase of Paper chromatography: water trapped between the fibres in the paper.
Stationary phase of TLC: thin coating of absorbent solid on a sheet of plastic or glass. Silica,
alumina or cellulose.
Mobile phase of TLC: ethanol and NH3
Mobile phase of paper chromatography: ethanol and NH3
Chromatography solvent: butan-1-ol: ethanoic acid: water
Paper chromatography and TLC are both examples of qualitative analysis. Paper
chromatography can be revealed using UV light or by developing the chromatogram by
revealing it to a gas or vapour which gives the compound colour when it is reacted with paper.
TLC determines in a mixture the number of components which allows 2 substances to be
identified or it monitors the reaction’s progress.
In both TLC and Paper chromatography, the substance that is present in the chromatography is
shown by how intense the coloured spot is.
Thin Layer Chromatography of amino acids
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