Exam (elaborations)
ACS – Biochemistry: Questions With Solutions
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ACS – Biochemistry: Questions With Solutions
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ACS – Biochemistry: Questions With Solutionsnet reaction for glycolysis Right Ans - 2x Pyruvate + 2x ATP + 2x NADH per
glucose molecule
what step produces 2x NADH in glycolysis? Right Ans - G3P -> 1,3 BPG
What enzyme/pathway does ATP inhibit? Right Ans - A high level of ATP
inhibits the enzyme PFK-1 by decreasing its affinity for fructose 6-phosphate.
AMP diminishes and citrate enhances the inhibitory effect of ATP.
The NADPH produced by HMP is used for Right Ans - reductive
biosynthesis (anabolic)
how do competitive inhibitors affect Km and Vmax? Right Ans - increases
Km, doesn't change Vmax
how do noncompetitive inhibitors affect Km and Vmax? Right Ans - Km
doesn't change, Vmax lowers
SDS-PAGE Right Ans - Type of chromatography used to separate proteins
based on mass. SDS binds to the proteins, giving them a negative charge
cancelling the effect of charges from the individual amino acids. Lighter
proteins travel faster than heavier ones.
PCR Right Ans - polymerase chain reaction- DNA is copied multiple times
to produce many copies of the original molecules helpful when there's only a
small DNA sample
Arginine (pka) Right Ans - Positively charged R groups (pKa=12.5)
Aspartic Acid (pka) Right Ans - Negatively charged R groups (pKa=3)
Cysteine (pka) Right Ans - Polar, uncharged R groups (pKa=8.3)
Glutamic Acid (pka) Right Ans - Negatively charged R groups (pKa=4)
Histidine (pka) Right Ans - Positively charged R groups (pKa=6.0)
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