ACS – Biochemistry: Questions With Solutions

ACS – Biochemistry: Questions With Solutions

net reaction for glycolysis Right Ans - 2x Pyruvate + 2x ATP + 2x NADH per
glucose molecule

what step produces 2x NADH in glycolysis? Right Ans - G3P -> 1,3 BPG

What enzyme/pathway does ATP inhibit? Right Ans - A high level of ATP
inhibits the enzyme PFK-1 by decreasing its affinity for fructose 6-phosphate.
AMP diminishes and citrate enhances the inhibitory effect of ATP.

The NADPH produced by HMP is used for Right Ans - reductive
biosynthesis (anabolic)

how do competitive inhibitors affect Km and Vmax? Right Ans - increases
Km, doesn't change Vmax

how do noncompetitive inhibitors affect Km and Vmax? Right Ans - Km
doesn't change, Vmax lowers

SDS-PAGE Right Ans - Type of chromatography used to separate proteins
based on mass. SDS binds to the proteins, giving them a negative charge
cancelling the effect of charges from the individual amino acids. Lighter
proteins travel faster than heavier ones.

PCR Right Ans - polymerase chain reaction- DNA is copied multiple times
to produce many copies of the original molecules helpful when there's only a
small DNA sample

Arginine (pka) Right Ans - Positively charged R groups (pKa=12.5)

Aspartic Acid (pka) Right Ans - Negatively charged R groups (pKa=3)

Cysteine (pka) Right Ans - Polar, uncharged R groups (pKa=8.3)

Glutamic Acid (pka) Right Ans - Negatively charged R groups (pKa=4)

Histidine (pka) Right Ans - Positively charged R groups (pKa=6.0)