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Biochemistry ACS Final Exam – Questions/Answers
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Biochemistry ACS Final Exam – Questions/Answers
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Biochemistry ACS Final Exam – Questions/AnswersWhat is the Henderson-Hasselbach ? Right Ans - pH = pka + log ([A]/[Ha])
High Ka, low pka indicates what? Right Ans - Strong acid
What is the relation between pH and [H+]? Right Ans - pH = -log[H+]
What is the realtionship between pka and Ka? Right Ans - pka = -logKa
What makes a good buffer? Right Ans - A weak acid mixed with its
conjugate base in a 50/50 ratio
What destroys tryptophan? Right Ans - Acids and bases
What is the isoelectric point? Right Ans - The pH in which a particular
molecule carries no net charge (pI)
Side chain pka of aspartic acid? Right Ans - ~ 3.86
Side chain pka of arginine? Right Ans - ~ 12.48
Side chain pka of cysteine? Right Ans - ~8.0
Side chain pka of glutamic acid? Right Ans - ~4.07
Side chain pka of histidine? Right Ans - ~6.1
Side chain pka of lysine? Right Ans - ~10.53
Side chain pka of tyrosine? Right Ans - ~10.00
Inductive effect on pka? Right Ans - Electronegative atoms pull electrons
away from another group, and makes that particular groups more positive.
Generally lowers pka
Electrostatic effect on pka? Right Ans - Nearby positive charge makes a
proton want to leave more, generally lowers pka.
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