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Biochemistry ACS – Questions & Complete Answers
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Biochemistry ACS – Questions & Complete Answers
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Biochemistry ACS – Questions & Complete AnswersDNA Right Ans - carry genetic information, double-stranded, more stable
RNA Right Ans - intermediate of gene expression, single-stranded, less
stable
tRNA Right Ans - transfer RNA, holds amino acid for protein synthesis
wobble position Right Ans - looser binding, pairing of 3rd codon less
critical
inosine Right Ans - closely resembles G, can pair with A, U, or C, allows
tRNA flexibility
rRNA Right Ans - ribosome RNA, makes up ribosomes
siRNA Right Ans - small interfering RNA, double-stranded, mRNA
degradation
miRNA Right Ans - micro RNA, silencing and post-transcriptional
regulation
ribozyme Right Ans - ribosomes catalyze peptide bond formation
purines Right Ans - 2 rings, adenine(NH), guanine (O)
pyramidines Right Ans - 1 ring, cytosine (NH), uracil (O), thymine (CH3)
A-T/U Right Ans - 2 H bonds
G-C Right Ans - 3 H bonds
Tm Right Ans - temp at which 50% of DNA is annealed, GC higher than AT
A DNA Right Ans - short and wide, 11bp, cylindrical, dehydrated, right
handed, angled base pairs, weaker H bonds
B DNA Right Ans - 10bp, right handed, parallel base pairs, strong H bonds
, Z DNA Right Ans - long and narrow, 12bp, left handed, GC rich
REN Right Ans - restriction endonuclease recombination
PCR Right Ans - polymerase chain reaction, denaturing (95C), anneal (65C),
extend (72C)
Sanger sequencing Right Ans - dideoxyNTP (ddNTP), causes DNA
polymerase to stop, color labeled
Helicase Right Ans - unwind, ATP dependent
single stranded binding protein Right Ans - prevents reannealing
DNA poly I Right Ans - removes primers
DNA poly II Right Ans - repairs
DNA poly III Right Ans - polymerization
DNA poly α Right Ans - initiation
DNA poly δ Right Ans - lagging
DNA poly ε Right Ans - leading
DNA poly γ Right Ans - mitochondrial replication
DNA poly β Right Ans - repair
RNAse Right Ans - degrades RNA
ligase Right Ans - combines Okazaki fragments
telomerase Right Ans - adds telomeres
semi-conservative Right Ans - 1 template, 1 daughter
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