Exam (elaborations)
BIOL 151 Exam 4 Questions answers latest update
- Course
- BIOL 151
- Institution
- University Of Wisconsin - Madison
BIOL 151 Exam 4 Questions answers latest update
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BIOL 151 Exam 4List the three phases of DNA replication - Initiation
Elongation
Termination
All DNA polymerases require a short strand of DNA or RNA, called a ________, to
begin their synthesis. - primer
1' sugar carbon atom is attached to - nitrogenous base
3' sugar carbon atom is attached to - OH group
5' sugar carbon atom is attached to - phosphate group
The experiments with radioactively labeled phage that showed the DNA was the
genetic material were performed by - Hershey and Chase
The purpose of the Hershey Chase experiments was to determine whether a
bacteriophage injected ________ or ________ into bacteria. - Protein; DNA
,Place the steps of the experiment that Hershey and Chase performed to
demonstrate that DNA was the genetic material of bacteriophages in the correct
order. - 1. Bacteriophages were grown on media containing radioactive material
2. Bacteriophages were allowed to infect bacteria
3. A blender was used to remove bacteriophages from cell surfaces
4. The sample was centrifuged
5. Radioactivity was measured
Of the following lest, which 3 items are required for DNA replication - template
polymerase
nucleotides
The enzyme DNA _______ covalently links nucleotides to synthesize new DNA
strands together during DNA replication. - polymerase
The model organism used by Meselson and Stahl was - Escherichia coli
DNA replication that leads to the production of double helices with one parental
strand and one newly synthesized strand is consistent with - semiconservative
replication
Griffith's experiments were important because they showed - that the genetic
material could be passed from one cell to another
, An Okazaki fragment is a short fragment of DNA created on the _______ strand of
DNA - lagging
leading strand - synthesized continuously
lagging strand - synthesized in small fragments that are later connected
What class of enzymes relieve torsional strain in DNA strands ahead of the
replication fork? - Topoisomerase
When a double stranded DNA helix is unwound, two single strands of DNA are
formed. These strands have to be stabilized because their hydrophobic bases are
exposed to water. The proteins that stabilize the two single strands are called -
single-strand binding proteins
Adjacent nucleotides in the same DNA strands are held together by covalent
bonds known as __________ bonds. - phosphodiester
Nucleotides contain a sugar, a phosphate, and a nitrogenous _______ - base
Which DNA polymerase removes and replaces RNA primer segments in the
synthesis of the lagging strand because it had 5' to 3' exonuclease activity - I
Helicase - unwinds the double helix
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