Exam (elaborations)
BIO 344 Final Exam Questions answers latest update
- Course
- Bio 344
- Institution
- University Of Texas - Austin
BIO 344 Final Exam Questions answers latest update
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BIO 344 Final ExamWhat process allows scientists to "knock-down" genes? - RNAi analysis
What process allows scientists to determine the expression patterns (temporal
and spatial) of many genes at once? - DNA microarray
T/F: 2-D gel electrophoresis is a technique that can be used to study protein-
protein interactions. - False
DNA microarrays can be used to study ___. - The clustering of expression of genes
in time and space
T/F: Class II promoters can be identified using ChIP-chip analysis with an anti-TAF1
antibody. - True
T/F: ChIP-chip analysis can be used to identify DNA-binding sites for activators and
other proteins. - True
T/F: Mass spectrometry is not a quantitative method, so it has been difficult to
use it to analyze the expression levels of proteins. - True
,T/F: Yeast two-hybrid is a traditional technique used to probe protein-DNA
interactions. - False
T/F: SNPs or single nucleotide polymorphisms may help physicians design patient
specific drug treatment programs. - True
Mapping depends on a set of landmarks that can be used for ___. - Positional
cloning
Restriction fragment length polymorphisms (RFLP) - Landmarks with lengths of
restriction fragments given by a specific enzyme that vary from one individual to
another
___ use a special vector to help clone exons only. - Exon traps
CpG islands - DNA regions containing unmethylated CpG sequences
What information can be gleaned from genome sequence? - - Location of exact
coding regions for all the genes
- Spatial relationships among all the genes and exact distances between them
How is a coding region recognized? - - Contains an ORF long enough to code for a
phage protein
- Phage or bacterial ORF is the same as a gene's coding region
, ORF must: - - Start with ATG triplet
- End with stop codon
The base sequences of viruses and organisms that have been obtained range
from: - - Phages
- Bacteria
- Animals
- Plants
Comparison of the genomes of closely related and more distantly related
organisms can shed light on the ___ of these species. - Evolution
In ___, geneticists started to map and ultimately sequence the entire human
genome. - 1990
Shotgun sequencing - - Clones sequenced randomly
- Sequences would be pieced together using computer programs
Two high-capacity vectors have been used extensively in the Human Genome
Project: - - Mapping was done mostly using the yeast artificial chromosome,
which accepts a million base pairs
- Sequencing was done with bacterial artificial chromosomes accepting about
300,000 bp
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