BMS 6303 Clinical Microbiology Final
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Fall/Spring Semester Graded A
The microbiology laboratory receives a genital culture from a 28-year-old female. The
specimen direct Gram stain reveals, gram-positive cocci in clusters and gram-
negative cocci in chains. The microbiologist inoculates a sheep blood agar plate, a
chocolate agar plate, a modified thayer martin plate and a LIM Broth (selective
enrichment for group B streptococci). The second shift microbiologist identifies the
discrepancy and must take corrective action. The discrepancy indicates:
the preliminary Gram stain was erroneously reported.
The agency that published guidelines known as Standard Precautions to reduce the
risk of disease transmission in laboratories is
CDC.
Primary plating media for stool include
BA, Mac, HE, Campy
Class II biological safety cabinets
All of the above are correct
,Certain procedures require the use of a biological safety cabinet. These include
all of the above.
Specimens for microbiologic studies should be
All of the above are correct.
Holding media such as Stuart's medium and Amie's medium are used
to maintain the viability of microorganisms present in a specimen.
Which type of media is designed to support the growth of a wide range of
microorganisms?
Nutritive
(A) True or (B) False: Reporting or formal documentation of an accident in the lab must
be done FIRST before the healthcare worker receives immediate medical care.
False
The method of choice for sterilizing items such as antibiotic solutions, chemicals,
radioisotopes, and vaccines that are heat-sensitive is:
,filtration.
To be sterilized in an autoclave, media or instruments should be:
processed for 15 minutes at 121°C and 15 psi pressure.
Which type of media is designed to support the growth of one group of organisms, but
not another, by containing antimicrobials, dyes, or alcohol?
Selective
The appropriate storage for a cerebral spinal fluid specimen is ____°C.
37
A principal role of the clinical microbiologist includes:
quickly and accurately communicating information about the patient in both verbal and
written formats.
Routine microbiology cultures should include all of the following media except:
highly selective specialized media such as Löwenstein-Jensen agar.
, Selection of culture media for specific specimens includes a variety of media for all of
the following reasons except:
a back-up broth is included to provide inoculum for additional testing.
The microbiologist receives a sputum specimen that upon preliminary Gram staining
review of direct smear, contains numerous squamous epithelial cells and an excessive
amount of contaminating normal flora. Based on the microbiologists knowledge, the
microbiologist rejects the specimen and requests a new sample. The physician insists
that the microbiologist culture the sample as received. The microbiologist should:
set-up the culture as ordered noting the lack of specimen integrity that may
compromise culture results.
A definitive identification of a pathogen typically includes:
a confirmatory set of reactions that correlates to the preliminary characteristics.
The macroscopic examination for receipt and review of specimens should include all of
the following:
leakage, blood or mucus present, viscosity for fluids, color and consistency.
An example of a b-hemolytic gram-positive organism is:
Escherichia coli.
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