Microbiology Lab Exam 1 Review
Questions And Answers.
simple microscope - Verified ANS ✔✔ - a microscope that contains only one
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lens
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compound microscope - Verified ANS ✔✔ - microscope that allows light to pass
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through a specimen and uses two lenses to form an image
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field of view - Verified ANS ✔✔ - how much of the specimen you can see
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depth of field - Verified ANS ✔✔ - how much of a vertical area remains in focus
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working distance - Verified ANS ✔✔ - how far the objective is from the slide
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low power objective - Verified ANS ✔✔ - provides a magnification of 10x and is
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the shortest objective
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high-dry objective - Verified ANS ✔✔ - 40x; blue line; equipped with a phase
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ring allowing phase contrast microscopy;
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oil immersion objective - Verified ANS ✔✔ - 100x; white line; most widely
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used; used w/oil that makes a continuous bridge b/w tip of objective and slide
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uses of oil in oil immersion objective - Verified ANS ✔✔ - allows more light to
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enter lens; improves resolution
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refractive index - Verified ANS ✔✔ - the speed of light in a given medium
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compared to the speed of light in a vacuum
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refracted - Verified ANS ✔✔ - light being bent as it passes into a medium of
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different density
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contrast - Verified ANS ✔✔ - ability to distinguish an image against the
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background
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, magnification - Verified ANS ✔✔ - the amount the image is enlarged; As Mag
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increases- field of view decreases-working distance decreases-amt of light
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decreases-depth of field decreases
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resolution - Verified ANS ✔✔ - - level of detail that can be observed
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brightfield microscopy - Verified ANS ✔✔ - just using the microscope; staining
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usually; adjusting iris diaphragm for better contrast
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darkfield microscopy - Verified ANS ✔✔ - used to get excellent contrast for live
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unstained specimens; bright with black background; most of the light coming
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from condensor misses objective, only light that passes through the specimen
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and is refracted passes into objective giving the bright image
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Phase-contrast microscopy - Verified ANS ✔✔ - specimen is dark on a light ml ml ml ml ml ml ml ml ml ml ml ml ml ml
background; used to increase quality of the image instead of bright field
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microscopy of unstained specimens; use a phase annular diaphragm under the
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condenser and a centering telescope
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Fluorescence Microscopy - Verified ANS ✔✔ - bright colored specimen on black
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background; absorption of light at one wavelength and emission of light at
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another; used in performing direct counts of bacteria
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diatoms - Verified ANS ✔✔ - produce cell walls of silica that resist decay and is
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mined for many uses
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heterocysts - Verified ANS ✔✔ - cyanobacteria that perform nitrogen fixation
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Properties of Algae and Cyanobacteria - Verified ANS ✔✔ - • Prominently (but
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not always) inhabit aquatic environments
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• Growth limited by amounts of available Phosphorus and Nitrogen
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• can form blooms if abundant (toxins)
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• Can be free-living or involved in symbioses
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• Carry out oxygenic photosynthesis
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Properties of Algae - Verified ANS ✔✔ - • Eukaryotic
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• Reproduction: sexual (most) and asexual (some)
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