MICROBIOLOGY LAB UTA - FINAL EXAM QUESTIONS AND CORRECT
DETAILED ANSWERS |ALREADY GRADED A+ LATEST UPDATE | 2024-2
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5 g. T.S.A. and 125 mL Di
water, cover flask with foil,
affix autoclave tape, auto-
How is Media made? clave, wait 10-20 min to
cool, pour into petri-dish
to thickness of lid. or stop
when covers bottom.
linear polysaccharide poly-
Agar mer derived from agarose
in red algae.
increases solidity, exhibits
hysteresis- gels and melts
What does agar do? at different temps. Melts
above 85-90 and solidifies
at 40-45.
commonly called broths,
doesn't contain agar, easy
What is liquid media?
to use, allows observation
of growth patterns.
Advantages to using To test oxygen require-
semi-solid media ments, to test motility.
only grows in a fortified
culture media under spe-
, MICROBIOLOGY LAB UTA - FINAL EXAM QUESTIONS AND CORRECT
DETAILED ANSWERS |ALREADY GRADED A+ LATEST UPDATE | 2024-2
Study online at https://quizlet.com/_g857zh
cific conditions. The more
_____________ the or-
ganism, the more specif-
Fastidious
ic ingredients a medium
must have. Uses defined
media.
The maintenance and
growth of stock cultures, to
Why use solid media?
isolate single colonies, to
ensure purity.
Tryptic Soy Agar most
common medium used in
lab. Undefined media that
T.S.A. Plate
contains casein digest,
soybean digest, NaCl, and
agar.
What are the 4 biochemi-
Proteins, Carbohydrates,
cal families all living things
lipids, nucleic acids
are composed of?
How much water does a
70%
bacteria cell consist of?
sterilizes with steam at
Autoclave
121* C, pressure at 15 PSI
, MICROBIOLOGY LAB UTA - FINAL EXAM QUESTIONS AND CORRECT
DETAILED ANSWERS |ALREADY GRADED A+ LATEST UPDATE | 2024-2
Study online at https://quizlet.com/_g857zh
for at least 15 min. Tested
weekly to daily.
potential source of infec-
Reservoir
tion.
S. pneumoniae and s. au- Normal commensal sym-
reus bionts
p. aeruginosa and b. cepa- Environmentally acquired
cia microbes
scanning: 4x-40, low:
Magnifications of our mi-
10x-100, high: 40x-400, oil
croscopes
immersion: 100x-1000
How close two objects
What is resolving power? can be and still be distin-
guished as separate.
What is the optimum res-
olution of the microscope .2 micrometer.
using oil immersion?
How much does each mi-
$1600
croscope cost?
convex
Morphology of a domed
colony
, MICROBIOLOGY LAB UTA - FINAL EXAM QUESTIONS AND CORRECT
DETAILED ANSWERS |ALREADY GRADED A+ LATEST UPDATE | 2024-2
Study online at https://quizlet.com/_g857zh
flatter dome than convex raised
flat elevation
crateriform
hole in convex colony
Umbonate
Morphology of a colony
with a raised center
Rhizoid
Morphology of a branching
colony
lobate
margin with lobes
irregular but not lobed undilated margins
wave pattern on margin curled
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