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BIOC 3021 Workshop 2 Questions and Answers 100% Solved | Graded A+ $9.49
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BIOC 3021 Workshop 2 Questions and Answers 100% Solved | Graded A+

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  • BIOC 3021

BIOC 3021 Workshop 2 Questions and Answers 100% Solved | Graded A+ Why does a plot of V vs S taper off and eventually reach a plateau at higher S levels? - - When S levels are high, the M-M plot actually approaches a limiting value of Vmax. - At high (S), where V approximates Vmax, virtually a...

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  • December 7, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • BIOC 3021
  • BIOC 3021
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BIOC 3021 Workshop 2 Questions and

Answers 100% Solved | Graded A+


Why does a plot of V vs S taper off and eventually reach a plateau at

higher S levels? - ✔✔- When S levels are high, the M-M plot actually

approaches a limiting value of Vmax.

- At high (S), where V approximates Vmax, virtually all of the enzyme active

sites are occupied by substrate.

- The addition of more S would not significantly increase the rate of

reaction, because the rate of reaction is limited by the total active sites

available; once all the sites are filled, the reaction can go no faster.

What is the Michaelis-Menten Equation? - ✔✔V = Vmax[S]/ [S] +km

The M-M Equation uses the terms (V), (Vmax), (S) and (Km). What do

each of these terms mean? - ✔✔V= observable velocity of the reaction.

Tells us how rapidly product (p) is being formed by the reaction.

Vmax = fastest reaction rate possible under the given assay conditions.

Has dimensions of change in product concentration per unit time.

S = substrate concentration


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©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.

, Km = Michaelis Constant. Has units of concentration

What does the Km term tell us about an enzyme? - ✔✔The Km tells us how

efficient enzymes are at low substrate levels

What does it signify if an enzyme has a low Km or a high Km? - ✔✔- The

Km tells us how efficient enzymes are at low substrate levels?

- At low Km, the enzyme is very efficient at low substrate concentration. At

high Km, enzymes are inefficient at low substrate concentration.

What is the enzyme rate when the concentration of S = Km? - ✔✔1/2

Vmax.

Why is a V vs S plot linear at low S concentrations? - ✔✔The M-M equation

reduces to V = K[S] which is the equation for a straight line.

This is b/c when Km is much greater than [S], the [S] term can be dropped

from the denominator

Why does the plot curve off at intermediate S levels - ✔✔Because the

whole M-M equation is used to describe the rate of the reaction.

Why does V vs S plot plateau at high S levels? - ✔✔The M-M equation

reduces to V=Vmax. When S levels are high, the M-M plot approaches a

limiting value of Vmax.




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©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.

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