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Biochemistry-Protein Synthesis and Protein Function

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  • Biochemistry
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  • Biochemistry

Dive into the fascinating world of molecular biology with this comprehensive textbook

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  • December 8, 2024
  • 8
  • 2024/2025
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  • Biochemistry
  • Biochemistry
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GoldenChapter
Biochemistry

Protein Synthesis And Protein Function




Compiled By Simon Mwangi
Edition: 2024/25

, Science | Biochemistry I of VII pages
1. what do ribosomes contain?
multimeric structure constituting of >50 proteins and one copy of each of 4 rRNAs (18S, 5.8S, 28S, 5S)

2. ribosomes are assembled in the
nucleus within the nucleolus

3. unlike other organelles, the nucleolus is not a
membrane bound structure

4. Small nucleolar RNAs (snoRNAs) serve as
guide
RNAs to direct specific modifications of the rRNAs

5. these modifications include (2)
about 100 methylations of the 2-OH position on the nucleotide ribose sugar and 100 isomerizations of uridine

6. Free ribosomes
move anywhere in the cytosol, but not found in the nucleus and other organelles

7. membrane bound ribosomes
if the protein being made contains an Endoplasmic Reticulum (ER) targeting sequence, then the ribosome is
with the rough ER. These types of proteins are transported to their destination through a secretory pathway

8. mRNA is read by the ribosomal machine as a
triplet of sequential nucleotides (called a codon)

9. Translation starts at the end of the mRNA
5

10. tRNAs are charged by the addition of
a specific amino acid that corresponds to that codon

11. his aminoacyl-tRNA is
created by the action of enzymes called
aminoacyl-tRNA
synthetases

12. Protein translation uses base pairing between the mRNA codon and a triplet complementary sequence in
anticodon

13. steps of translation (4)
activationinitiationelongationtermination

14. activation
formation of aminoacyl-tRNAs

15. initiation


Biochemistry 2024/25 Edition

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