Microbiology Lab Exam Study Guide Questions with Complete Solutions
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Course
Microbiology Lab
Institution
Microbiology Lab
Microbiology Lab Exam Study Guide
Questions with Complete Solutions
What is the purpose of aseptic technique in microbiology?
To prevent contamination of microbial cultures and the environment.
What type of bacteria will not grow on a MacConkey agar plate?
Gram-positive bacteria.
What i...
Microbiology Lab Exam Study Guide
Questions with Complete Solutions
What is the purpose of aseptic technique in microbiology?
✔✔To prevent contamination of microbial cultures and the environment.
What type of bacteria will not grow on a MacConkey agar plate?
✔✔Gram-positive bacteria.
What is the primary function of a Gram stain?
✔✔To differentiate bacteria into two groups: Gram-positive and Gram-negative.
What is the function of the bacterial capsule?
✔✔To protect the bacterium from phagocytosis and enhance its virulence.
How is an antibiotic susceptibility test performed?
✔✔By placing antibiotic-impregnated discs on an agar plate inoculated with the microorganism.
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,What does a positive result in a catalase test indicate?
✔✔The presence of catalase enzyme, which breaks down hydrogen peroxide.
What is a pure culture?
✔✔A culture containing only one species of microorganism.
What is the purpose of an inoculating loop in microbiology?
✔✔To transfer microorganisms from one medium to another.
What type of bacteria produce endospores?
✔✔Bacillus and Clostridium species.
What is the role of the ribosome in a bacterial cell?
✔✔To synthesize proteins from RNA.
What is the difference between an obligate aerobe and an obligate anaerobe?
✔✔An obligate aerobe requires oxygen, while an obligate anaerobe cannot tolerate oxygen.
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, What does an oxidase test determine in microbiology?
✔✔It identifies bacteria that produce cytochrome c oxidase.
What is the function of agar in microbiological media?
✔✔To solidify the medium and provide a surface for microbial growth.
What is the purpose of performing a streak plate?
✔✔To isolate individual bacterial colonies from a mixed culture.
What is the role of the bacterial cell membrane?
✔✔To regulate the movement of substances into and out of the cell.
What are obligate intracellular parasites?
✔✔Microorganisms that can only replicate inside a host cell.
What is the difference between a virus and a bacterium?
✔✔Viruses are not cellular and require a host to replicate, while bacteria are cellular organisms.
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