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Microbiology Lab Exam 1 Questions and Answers Graded A+
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Microbiology Lab Exam 1 Questions and Answers Graded A+ What does a positive result in a TSI (Triple Sugar Iron) test look like for glucose fermentation? The butt of the tube turns yellow, indicating glucose fermentation and acid production. What is the result of a positive test for ureas...

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  • December 9, 2024
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  • 2024/2025
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Microbiology Lab Exam 1 Questions and
Answers Graded A+
What does a positive result in a TSI (Triple Sugar Iron) test look like for glucose fermentation?


✔✔The butt of the tube turns yellow, indicating glucose fermentation and acid production.




What is the result of a positive test for urease activity?


✔✔The medium turns pink, indicating that the bacterium produces urease and hydrolyzes urea.




What does a positive catalase test look like?


✔✔Bubbles appear when hydrogen peroxide is applied to the bacterial culture, indicating the

presence of catalase.




What is the appearance of a positive phenol red sugar fermentation test?


✔✔The medium changes color to yellow, indicating acid production from sugar fermentation.




What is the significance of the coagulase test?


✔✔It is used to identify Staphylococcus aureus by its ability to coagulate plasma.




1

,What is the purpose of the bile esculin test?


✔✔To differentiate Enterococcus species from other Gram-positive cocci based on their ability

to hydrolyze esculin in the presence of bile.




What does a positive Voges-Proskauer test show?


✔✔A red color develops, indicating the bacterium ferments glucose to acetoin, which is further

metabolized to a neutral product.




What does a positive nitrate reduction test show?


✔✔A red color develops, indicating that nitrate has been reduced to nitrite or nitrogen gas by the

bacterium.




What does a positive starch hydrolysis test show?


✔✔A clear zone around the bacterial growth indicates that the bacterium produces amylase and

can hydrolyze starch.




What is the purpose of a Gram stain in microbiology?


✔✔To differentiate bacterial species into Gram-positive or Gram-negative based on the cell wall

structure.

2

,What is the difference between a positive and negative catalase test?


✔✔A positive catalase test produces bubbles when hydrogen peroxide is applied, indicating the

presence of catalase enzyme.




What does a positive result in a coagulase test indicate?


✔✔It indicates that the bacterium produces coagulase, which causes blood plasma to clot.




What is the function of bacterial pili?


✔✔Pili are used for adhesion to surfaces and genetic exchange during conjugation.




What is a characteristic feature of Gram-positive bacteria?


✔✔They have a thick peptidoglycan layer that retains the crystal violet stain, appearing purple

under the microscope.




What does a positive result for an oxidase test look like?


✔✔A positive result produces a purple color within seconds, indicating the presence of

cytochrome c oxidase.



3

, What is the appearance of a positive phenol red fermentation test?


✔✔The medium changes from red to yellow, indicating acid production from fermentation.




What does a positive result in a motility test indicate?


✔✔The bacterium is able to move throughout the medium, causing cloudiness beyond the

inoculation line.




What is the purpose of a MacConkey agar plate?


✔✔To differentiate bacteria based on their ability to ferment lactose and to select for Gram-

negative bacteria.




What is the result of a positive starch hydrolysis test?


✔✔A clear zone around the bacterial growth indicates the hydrolysis of starch by amylase.




What does a positive methyl red test indicate?


✔✔The bacterium performs mixed-acid fermentation, lowering the pH and turning the medium

red.



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