Microbiology Lab Chapter 3 – Questions and Answers Graded A+
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Microbiology Lab Chapter 3 –
Institution
Microbiology Lab Chapter 3 –
Microbiology Lab Chapter 3 – Questions
and Answers Graded A+
What does a positive result in a TSI (Triple Sugar Iron) test indicate when both glucose and
sucrose are fermented?
Yellow slant and yellow butt, indicating acid production from glucose and sucrose
fermentation.
What is th...
Microbiology Lab Chapter 3 – Questions
and Answers Graded A+
What does a positive result in a TSI (Triple Sugar Iron) test indicate when both glucose and
sucrose are fermented?
✔✔Yellow slant and yellow butt, indicating acid production from glucose and sucrose
fermentation.
What is the result of a positive test for urease activity?
✔✔A pink color forms, indicating that the bacterium produces urease and hydrolyzes urea.
What is the characteristic appearance of a positive phenol red fermentation test?
✔✔The medium changes from red to yellow, indicating acid production from sugar
fermentation.
What is the purpose of a catalase test?
✔✔To determine if a bacterium produces catalase, which breaks down hydrogen peroxide into
oxygen and water.
1
, What does a positive methyl red test result look like?
✔✔A red color develops, indicating that the bacterium performs mixed-acid fermentation,
lowering the pH.
What is the appearance of a positive bile esculin test?
✔✔The medium turns black, indicating that the bacterium can hydrolyze esculin in the presence
of bile.
What is the result of a positive gelatin hydrolysis test?
✔✔The medium becomes liquefied, indicating the bacterium produces gelatinase.
What is the primary purpose of a motility test?
✔✔To determine if a bacterium is capable of moving through the medium.
What is the significance of a positive VP (Voges-Proskauer) test?
✔✔A red color develops, indicating the production of acetoin during fermentation.
What does a positive oxidase test indicate?
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