Microbiology Lab Midterm Questions and Answers Rated A+
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Microbiology Lab Mid term
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Microbiology Lab Mid Term
Microbiology Lab Midterm Questions
and Answers Rated A+
What is the purpose of the oxidase test?
To detect the presence of cytochrome c oxidase.
What is an acid-fast stain used for?
To identify bacteria with waxy cell walls, like Mycobacterium.
What color do acid-fast bacteria appea...
Microbiology Lab Midterm Questions
and Answers Rated A+
What is the purpose of the oxidase test?
✔✔To detect the presence of cytochrome c oxidase.
What is an acid-fast stain used for?
✔✔To identify bacteria with waxy cell walls, like Mycobacterium.
What color do acid-fast bacteria appear after staining?
✔✔Red or pink.
What is the main function of a bacterial capsule?
✔✔To protect the bacterium from phagocytosis.
What is the primary role of endospores in bacteria?
✔✔To ensure survival under harsh conditions.
1
,What is an example of a spore-forming bacterium?
✔✔Bacillus subtilis.
What is the purpose of using aseptic techniques in the microbiology lab?
✔✔To prevent contamination of cultures and the environment.
What is the primary function of an incubator in microbiology?
✔✔To maintain optimal temperature conditions for microbial growth.
What is the role of the streak plate method?
✔✔To isolate individual colonies from a mixed culture.
What is the most common stain used to differentiate bacterial cell walls?
✔✔Gram stain.
What type of bacteria stain purple in a Gram stain?
✔✔Gram-positive bacteria.
2
,What is the first reagent used in a Gram stain procedure?
✔✔Crystal violet.
What is the purpose of the iodine step in Gram staining?
✔✔To fix the crystal violet to the bacterial cell wall.
Why is alcohol used during the Gram stain procedure?
✔✔To decolorize Gram-negative cells.
What is the counterstain used in the Gram stain?
✔✔Safranin.
What is the shape of cocci bacteria?
✔✔Spherical.
What does it mean if a bacterium is described as bacillus?
✔✔It has a rod shape.
3
, What is the purpose of using agar in microbiological media?
✔✔To provide a solid surface for microbial growth.
What does a turbid liquid culture indicate?
✔✔The presence of microbial growth.
What is the purpose of using selective media?
✔✔To suppress the growth of certain microorganisms while allowing others to grow.
What is an example of a differential medium?
✔✔MacConkey agar.
What is the purpose of performing a catalase test?
✔✔To determine if an organism produces the enzyme catalase.
What result indicates a positive catalase test?
✔✔The production of bubbles when hydrogen peroxide is added.
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