Microbiology Lab Practical 1 Gacura Questions and Answers Graded A+
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Microbiology Lab Practical 1 Gacura
Institution
Microbiology Lab Practical 1 Gacura
Microbiology Lab Practical 1 Gacura
Questions and Answers Graded A+
What is the first step in preparing a bacterial smear for staining?
Place a small drop of water on the slide to suspend the bacteria.
Why is it important to heat-fix the bacterial smear?
To kill the bacteria, adhere them...
Microbiology Lab Practical 1 Gacura
Questions and Answers Graded A+
What is the first step in preparing a bacterial smear for staining?
✔✔ Place a small drop of water on the slide to suspend the bacteria.
Why is it important to heat-fix the bacterial smear?
✔✔ To kill the bacteria, adhere them to the slide, and prevent distortion during staining.
What is the purpose of the Gram stain in microbiology?
✔✔ To differentiate bacteria based on the structure of their cell walls, identifying Gram-positive
and Gram-negative bacteria.
What is the primary stain used in the Gram stain procedure?
✔✔ Crystal violet.
What is the purpose of the decolorizer in the Gram stain process?
✔✔ To remove the primary stain from Gram-negative bacteria, leaving them colorless.
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,What does a Gram-positive bacterium appear as after a Gram stain?
✔✔ Purple.
What color do Gram-negative bacteria appear after the Gram stain procedure?
✔✔ Pink or red.
What is the function of the counterstain in the Gram stain procedure?
✔✔ To stain the Gram-negative bacteria that were decolorized by the ethanol.
What is the role of the condenser on a microscope?
✔✔ To focus light onto the specimen, enhancing visibility.
Why should you never use the coarse focus knob when using the 100x objective lens?
✔✔ To avoid damaging the lens or the slide, as it could cause the objective lens to crash into the
slide.
What is the total magnification when using a 10x ocular lens and a 40x objective lens?
✔✔ 400x.
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, Why is oil immersion used with the 100x objective lens?
✔✔ To reduce light refraction and increase the resolution, allowing for better visibility of fine
details.
What is the purpose of using a petri dish in microbiology?
✔✔ To culture and isolate microorganisms on a solid medium.
What is a common use of MacConkey agar in microbiology?
✔✔ To isolate Gram-negative bacteria and differentiate lactose fermenters.
What does a positive result for lactose fermentation look like on MacConkey agar?
✔✔ Pink colonies due to acid production from lactose fermentation.
What is the purpose of the streak plate method?
✔✔ To isolate individual bacterial colonies from a mixed culture.
How should a Bunsen burner be lit in a microbiology lab?
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