Microbiology Lab Exam 1 Questions and Answers 100% Pass
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Course
Microbiology Lab
Institution
Microbiology Lab
Microbiology Lab Exam 1 Questions and
Answers 100% Pass
What is the purpose of using immersion oil with a 100x objective lens?
To reduce light refraction and improve resolution.
Why should you always start focusing a specimen with the lowest power objective?
To locate the specimen and ...
Microbiology Lab Exam 1 Questions and
Answers 100% Pass
What is the purpose of using immersion oil with a 100x objective lens?
✔✔ To reduce light refraction and improve resolution.
Why should you always start focusing a specimen with the lowest power objective?
✔✔ To locate the specimen and prevent damage to the slide.
What is the function of the condenser on a microscope?
✔✔ To focus and direct light onto the specimen.
How is total magnification calculated on a microscope?
✔✔ By multiplying the magnification of the objective lens by the magnification of the ocular
lens.
What is the purpose of the mechanical stage on a microscope?
✔✔ To hold the slide in place and allow precise movement.
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,Why is it important to use a clean lens for microscopy?
✔✔ To ensure clear and accurate visualization of specimens.
What is a bacterial smear, and why is it prepared?
✔✔ A thin layer of bacteria spread on a slide to observe cell morphology and structure.
What is the primary stain used in the Gram stain procedure?
✔✔ Crystal violet.
What does the decolorizer do in the Gram stain process?
✔✔ Removes the primary stain from Gram-negative bacteria.
Why are acid-fast bacteria difficult to stain using conventional methods?
✔✔ Their waxy cell walls resist penetration by aqueous stains.
What type of stain is used to differentiate fungal spores from vegetative cells?
✔✔ Lactophenol cotton blue.
2
,What is the purpose of a simple stain?
✔✔ To color cells and make their shape and arrangement more visible.
What is the defining feature of prokaryotic cells?
✔✔ They lack a membrane-bound nucleus and organelles.
What is the ecological role of nitrogen-fixing bacteria?
✔✔ They convert atmospheric nitrogen into a form usable by plants.
What is mycelium in fungi?
✔✔ A network of filamentous hyphae that forms the vegetative part of the fungus.
What are fungal hyphae, and what do they do?
✔✔ Thread-like structures that grow and absorb nutrients.
What distinguishes yeast from mold in fungi?
✔✔ Yeast is unicellular, while mold is multicellular and filamentous.
3
, What type of reproduction is typical of yeast?
✔✔ Asexual reproduction through budding.
What is the main role of fungi in ecosystems?
✔✔ Decomposition and nutrient recycling.
What are lichens, and how are they formed?
✔✔ Symbiotic associations between fungi and photosynthetic organisms like algae or
cyanobacteria.
What is a sporangium in fungi?
✔✔ A structure that produces and contains spores.
What is the purpose of using selective media in microbiology?
✔✔ To promote the growth of specific microorganisms while inhibiting others.
What is the main difference between prokaryotes and eukaryotes in terms of DNA?
✔✔ Prokaryotes have circular DNA, while eukaryotes have linear DNA within a nucleus.
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