Microbiology Lab Exam 2 Questions and Answers Already Passed
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Course
Microbiology Lab
Institution
Microbiology Lab
Microbiology Lab Exam 2 Questions and
Answers Already Passed
What is the counterstain used in the Gram stain procedure?
Safranin.
What is the purpose of heat-fixing a bacterial smear before staining?
To adhere the bacteria to the slide and kill the cells.
Which type of bacteria has ...
Microbiology Lab Exam 2 Questions and
Answers Already Passed
What is the counterstain used in the Gram stain procedure?
✔✔ Safranin.
What is the purpose of heat-fixing a bacterial smear before staining?
✔✔ To adhere the bacteria to the slide and kill the cells.
Which type of bacteria has a thick peptidoglycan layer in its cell wall?
✔✔ Gram-positive bacteria.
Why is it important to use fresh cultures for Gram staining?
✔✔ Old cultures may lose their ability to retain the primary stain, leading to inaccurate results.
What does the presence of an endospore indicate about a bacterial cell?
✔✔ That the bacterium is capable of surviving harsh environmental conditions.
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, What stain is used to visualize bacterial endospores?
✔✔ Malachite green.
What role does safranin play in endospore staining?
✔✔ It acts as a counterstain to color the vegetative cells.
What color do Gram-positive bacteria appear after a Gram stain?
✔✔ Purple.
What is an acid-fast stain used to detect?
✔✔ Bacteria with waxy cell walls containing mycolic acid, such as *Mycobacterium* species.
What is the primary stain used in the acid-fast staining technique?
✔✔ Carbol fuchsin.
What decolorizing agent is used in acid-fast staining?
✔✔ Acid-alcohol.
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