USMLE biochemistry- Exam Questions and Correct Answers Latest Version (2024-2025) Graded A+
Fatty acid oxidation, occurs in the mitochondria or cytsol? - Answers mitochondria (break down fatty
acids)
AcetylCoA production, cytosol or mito? - Answers mitochondria
TCA cycle, mitochondria or cytosol? - Answers mitochondira
Fatty acid synthesis, mitochondria or cytosol? - Answers cytosol
HMP shunt, located in mitochondria or cytosol? - Answers cytosol (next to PM)
steroid synthesis, mitochondria or cytoplasm? - Answers cytosol
What 3 metabolic processes occur in both the cytoplasm and mitochondria? - Answers Heme synthesis
Urea cycle
Gluconeogenesis
(HUG)
what co factor does carboylase use? - Answers biotin (buy-a-tin of CO2)
Phosphofructokinase-1 is the RLM for? - Answers Glycolysis
what are the+allosteric regulators of PFK-1 - Answers 1. AMP+, fructose-2,6-bisphosphate
what are the - allosteric regulators of PFK-1? - Answers ATP, CItrate
Fructose-1,6 Bisphosphatase is the RLM for what process? - Answers Gluconeogenesis
What are the + allosteric regulators of GNG? - Answers 1. ATP, AcetylCoA
What are the -negative allosteric regulators of GNG? - Answers AMP, F-2,6-BP
Isocitrate Dehydrogenase is the RLM enzyme for what process? - Answers TCA cycle
What are the allosteric regulators of isocitrate dehydrogenase? (give +/-) - Answers +: ADP
-: ATP, NADH
What disease has excess NADH leading to inhibition of TCA cycle via isocitrate dehydrogenase? -
Answers Alcoholism. Excess EtOH leads to increased NADH.
Glycogen synthetase is the RLM for what process? - Answers Glycogen synthesis
, What are the allosteric regulators of glycogen synthetase? - Answers +:Glucose-6-Phosphate, insulin,
cortisol
-:epinephrine, glucagon
Glycogen Phosphorylase is the RLM for what process? - Answers Glycogenolysis
What are the allosteric regulators of glycogen phosphorylase? - Answers +: epinephrine, glucagon, AMP
-:Glucose-6-Phosphate, insulin, ATP
What is the RLM of HMP shunt? - Answers Glucose-6-phosphate dehydrogenase
What is the RLM of de novo pyrimidine synthesis? - Answers Carbamoyl phosphate synthetase II
What is the RLM of de novo purine synthesis? - Answers PPRP
What is the RLM of the urea cycle? - Answers Carbamoyl phosphate I
Acetyl CO-A carboxylase is the RLM of what reaction? - Answers Fatty acid synthesis
Carnitine acyltransferase I is the RLM for what process? - Answers Fatty acid degredation
What is the RLM of ketogenesis? - Answers HMG CO synthase
What is the RLM of cholesterol synthesis - Answers HMG CO reductase
what are the allosteric regulators of HMG COaReductase? - Answers +: insulin, thyroxine
-:glucagon, cholesterol
Aerobic metabolism produces how many net ATP via the malate aspartate shuttle? - Answers 32
How many ATP produced via the glycerol 3-Phosphate shuttle? - Answers 30
How many ATP does anaerobic glycolysis produce? - Answers 2
arensic causes ATP production become what? - Answers 0
NAD+ is used in anabolic processes T/F? - Answers False, NAD+ is used in catabolic processes (think
glycolysis/TCA)
NADPH is used in anabolic processes T/F? - Answers True! steroid or fatty acid synthesis, provides
reducing equivalents.
Name the 4processes NADPH is involved in - Answers 1. Anabolic processes
2. respiratory burst
3. cytochrome p450 system
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