Microbiology Lab Practical – Final Exam
with Solutions
Staph epidermidis Correct Ans-This is MSA and the side labelled "2" is red. Which Staph
does this indicate?
Staph aureus Correct Ans-This is MSA and the side labelled "1" has turned yellow. Which
Staph does this indicate?
Lactose Negative Correct Ans-This is an EMB plate. What does the test result of "1"
indicate?
Lactose Positive Correct Ans-This is an EMB plate. What does the test result of "2" indicate?
E. Coli Correct Ans-This is an EMB plate. What is indicated by "3?"
Starch was broken down Correct Ans-This is a starch plate. What does the clear boundary
around this colony indicate?
acid was produced Correct Ans-Why did this Fermentation Tube turn yellow?
gas was produced Correct Ans-What is indicated by the bubble in this Durham Tube inside a
Fermentation Tube?
,Protein was not broken down Correct Ans-This is a Gelatin Tube Test. In Test Tube 2, it is
solid. What does this mean?
Catalase Test Correct Ans-What is the name of this test?
Antibiotic Disk Diffusion (Antibiotic Susceptibility) Correct Ans-What is the name of this
test?
Mannitol Salt Agar Correct Ans-What is the name of this media?
Urea Broth
Urease Enzyme
Tube 1: Positive
Tube 2: Negative Correct Ans-What is it testing for?
What does pink indicate?
What does the pale yellow indicate?
Nitrate Reduction Correct Ans-What is the name of this test?
neutrophil Correct Ans-
, lymphocyte Correct Ans-
monocyte Correct Ans-
eosinophil Correct Ans-
basophil Correct Ans-
What method is used for the antimicrobial susceptibility test? Correct Ans-Kirby-Bauer
What technique is used for the Kirby-Bauer test? Correct Ans-swab and make a bacterial
lawn
What is the principle of technique for the Kirby-Bauer? Correct Ans-disk diffusion
What observations are made in lab with Kirby-Bauer? Correct Ans-zone of inhibition
What is reported out to Physicians from the Kirby-Bauer? Correct Ans-susceptibility or
resistance
What is produced when UV radiation is absorbs by DNA? Correct Ans-pyrimidine dimers -
which interferes with DNA replication and transcription
with Solutions
Staph epidermidis Correct Ans-This is MSA and the side labelled "2" is red. Which Staph
does this indicate?
Staph aureus Correct Ans-This is MSA and the side labelled "1" has turned yellow. Which
Staph does this indicate?
Lactose Negative Correct Ans-This is an EMB plate. What does the test result of "1"
indicate?
Lactose Positive Correct Ans-This is an EMB plate. What does the test result of "2" indicate?
E. Coli Correct Ans-This is an EMB plate. What is indicated by "3?"
Starch was broken down Correct Ans-This is a starch plate. What does the clear boundary
around this colony indicate?
acid was produced Correct Ans-Why did this Fermentation Tube turn yellow?
gas was produced Correct Ans-What is indicated by the bubble in this Durham Tube inside a
Fermentation Tube?
,Protein was not broken down Correct Ans-This is a Gelatin Tube Test. In Test Tube 2, it is
solid. What does this mean?
Catalase Test Correct Ans-What is the name of this test?
Antibiotic Disk Diffusion (Antibiotic Susceptibility) Correct Ans-What is the name of this
test?
Mannitol Salt Agar Correct Ans-What is the name of this media?
Urea Broth
Urease Enzyme
Tube 1: Positive
Tube 2: Negative Correct Ans-What is it testing for?
What does pink indicate?
What does the pale yellow indicate?
Nitrate Reduction Correct Ans-What is the name of this test?
neutrophil Correct Ans-
, lymphocyte Correct Ans-
monocyte Correct Ans-
eosinophil Correct Ans-
basophil Correct Ans-
What method is used for the antimicrobial susceptibility test? Correct Ans-Kirby-Bauer
What technique is used for the Kirby-Bauer test? Correct Ans-swab and make a bacterial
lawn
What is the principle of technique for the Kirby-Bauer? Correct Ans-disk diffusion
What observations are made in lab with Kirby-Bauer? Correct Ans-zone of inhibition
What is reported out to Physicians from the Kirby-Bauer? Correct Ans-susceptibility or
resistance
What is produced when UV radiation is absorbs by DNA? Correct Ans-pyrimidine dimers -
which interferes with DNA replication and transcription
