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MCB 251 Final Exam Lon-Capa Questions And Answers With Verified Solutions Already Passed Latest Updated 2025 $15.99
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MCB 251 Final Exam Lon-Capa Questions And Answers With Verified Solutions Already Passed Latest Updated 2025
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MCB 251 Final Exam Lon-Capa Questions And Answers With Verified Solutions Already Passed Latest Updated 2025

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  • January 9, 2025
  • 12
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • mcb 251 final exam lon capa questions and answers
  • when measuring the band size of your unknown if y
  • you perform a pcr and unfortunately you see non sp
  • what is the purpose of loading dye

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MCB 251 Final Exam Lon-Capa
Questions And Answers With Verified
Solutions Already Passed Latest
Updated 2025
When measuring the band size of your unknown, if your band has traveled
further than all of the bands from the standard, what must you do to obtain
accurate results?
Answer: Run the gel again with a standard that has smaller bands

You perform a PCR and unfortunately you see non-specific amplification.
You go back to your protocol to decide what changes should be made. Which
of the following would you do?
Answer: Increase the annealing temperature

When performing a PCR and you see no products, which of the following is
least likely to yield a better product?
Answer: Decrease the number of cycles

What is the purpose of loading dye?
Answer:
To help samples sink into wells
To help track the progress of the DNA

You have a bacteria with a plasmid and you want to check to see if it contains
a specific gene of interest. What should you do?
Answer: Extract plasmid, PCR, check on gel

To increase the separation of the large sized bands in your DNA agarose gel,
you want to:
Answer: Decrease the % agarose

If the reverse primer is complimentary to itself, it can form:
Answer: Primer dimers

Your friend gives you a pUC-19 empty vector. Use the following vector map to
propose 2 tests to verify that the plasmid you have been given is actually pUC-
19.

, Answer:
Test 1: Transform plasmid onto E. coli cells using heat shock to take up the
plasmid. If the plasmid is there, it should grow on agar and ampicillin. Using
restriction enzyme BsmBI, it produces 54 base pairs and 2623 base pairs.
Test 2: This test is similar but using different restriction enzymes. Make sure to
always cut with two. Cut with AhDI and ScaI, and if the correct amount of base
pairs is produced, you know you have pUC-19.

When performing a transformation reaction using pBlu, you decide to also
add a negative control in which no vector was added to your competent cells
before heat shock. When plating onto LA Amp, you realize that the negative
control plate has 230 colonies. What is NOT a possible reason for this
unexpected growth?
Answer: You added too much antibiotic to the plate

After finishing your cloning project in which you insert GFP into pBLU, what
would NOT be a way in which you could check if your clones were correct?
Answer: Isolate the plasmid using a Qiagen kit and re-transform into another
background strain

What is the ideal ligation ratio of vector to insert?
Answer: 1 vector : 3 insert

What are the key characteristics of a good primer set?
Answer:
No secondary structure
Similar annealing temperature
3' end is a G or a C
No primer dimers

At 55 °C, taq DNA polymerase will:
Answer: Not elongate

The primer is elongated in which direction?
Answer: 5' to 3'

PCR primers are generally how long?
Answer: 15-30 bp

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