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Mcb101 Exam 1 (Lon-Capa Quiz 1,2,3,4,6) Questions And Answers With Verified Solutions 100% Correct Rated A+ $13.49
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Mcb101 Exam 1 (Lon-Capa Quiz 1,2,3,4,6) Questions And Answers With Verified Solutions 100% Correct Rated A+
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Mcb101 Exam 1 (Lon-Capa Quiz 1,2,3,4,6) Questions And Answers With Verified Solutions 100% Correct Rated A+

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  • January 9, 2025
  • 9
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

Subjects

  • mcb101 exam 1 lon capa quiz 12346 questions
  • streak plate vs spread plate
  • sterilization vs disinfection
  • increasing the following will improve the resoluti

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  • Capa certification
  • Capa certification

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Mcb101 Exam 1 (Lon-Capa Quiz
1,2,3,4,6) Questions And Answers With
Verified Solutions 100% Correct Rated
A+
Quiz 1: Aseptic Technique and Brightfield Microscopy
Streak Plate vs. Spread Plate
Streak Plate: Dilutes cells by mechanical separation on the media; Goal is to
generate pure culture.
Spread Plate: Dilutes cells prior to inoculating media; Goal is to determine
original CFU/mL.
Sterilization vs. Disinfection
Sterilization: Kills all microorganisms on a surface (e.g., Autoclave).
Disinfection: Kills many microorganisms on a surface (e.g., Bleach).
Increasing the following will improve the resolution of the image seen in the
microscope
Answer: Numerical aperture of the lens used to view the specimen.
Original concentration of the culture in CFU/mL
Answer: 19,500,000,000.
Why is it important to allow a sterilized inoculating loop to cool before
attempting to sample liquid culture?
Answer: To avoid creating aerosols.
In a properly conducted Gram stain, what color will Gram-negative cells be
immediately after treatment with safranin?
Answer: Pink.
Why does immersion oil improve the resolving power of the 100X objective?
Answer: Light diffracted by the specimen is directed into the 100X objective by
immersion oil.
When is a bacterial culture diluted using the streak plate technique?
Answer: As you streak.
Which of the following are characteristic of a positive/simple stain?
True: It stains all cells the same, It is positively charged and binds to the bacterial
cell wall.
False: It colors the background, leaving the bacterial cells clear or white, It is an
anionic stain, It is a differential stain because it stains cells differently than
negative stains.

, Why is it necessary to stain bacterial cells in order to view them using
brightfield microscopy?
True: Since bacterial cells are mostly water, they do not absorb much light;
Staining makes the cells more visible by increasing the contrast between the cell
and the background.
False: Staining is necessary in order to fix the cells to the slide; Staining always
gives us an accurate determination of shape; Only certain wavelengths of light are
detected by the objective. Staining ensures that certain wavelengths are reflected
by the specimen into the objective.

Quiz 2: Staining Techniques
What color are Gram-negative cells after they are stained?
Answer: Pink.
For a Gram-negative cocci, how would the cells appear after the following
steps of a Gram stain?
Crystal violet: Purple
Safranin: Pink
Iodine: Purple
Ethanol: Colorless
Bacterial cultures used for Capsule Stain are typically grown in milk-based
culture broths. Why?
Answer: Milk supplies the required carbohydrates.
Lack of heat-fixing during Negative Staining is both an advantage and a
disadvantage. How is lack of heat-fixing an advantage?
Answer: Able to visualize cells that cannot be stained by normal stains.
Acid-Fast stain for tuberculosis case - Was the stain performed properly?
Answer: Yes.
How do you know that the slide described in the previous question (acid-fast)
was stained properly?
Answer: The known Acid-Fast culture (Mycobacterium smegmatis) stained red.

Quiz 2: Staining Techniques

Which type of stain utilizes both positive and negative stains? - ANSWER✔✔
Capsule stain

Quiz 2: Staining Techniques

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