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UIUC MCB 450 EXAM 2 LON-CAPA PROBLEMS Questions And Answers With Verified Solutions Already Passed Latest Updated 2025 $14.99
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UIUC MCB 450 EXAM 2 LON-CAPA PROBLEMS Questions And Answers With Verified Solutions Already Passed Latest Updated 2025
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UIUC MCB 450 EXAM 2 LON-CAPA PROBLEMS Questions And Answers With Verified Solutions Already Passed Latest Updated 2025

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  • January 9, 2025
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  • 2024/2025
  • Exam (elaborations)
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  • uiuc mcb 450 exam 2 lon capa problems questions an
  • how does a nucleotide differ from a nucleoside
  • the major interaction stabilizing the double helic
  • which of the following is true regarding the relat

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UIUC MCB 450 EXAM 2 LON-CAPA
PROBLEMS Questions And Answers
With Verified Solutions Already Passed
Latest Updated 2025
1. How does a nucleotide differ from a nucleoside?
Answer: B. A nucleotide is a nucleoside with a phosphate ester linked to the sugar.
Explanation: A nucleotide consists of a nucleoside (which is made of a base and a
sugar) with one or more phosphate groups attached to the sugar.

2. The major interaction stabilizing the double helical structure of DNA is:
Answer: B. Hydrogen bonds between bases on the two strands.
Explanation: The primary interaction stabilizing the DNA double helix is the
hydrogen bonding between complementary base pairs (A-T, G-C).

3. Which of the following is TRUE regarding the relationship of replication
and transcription of DNA?
Answer: D. Replication requires both a template and a primer, while transcription
requires only a template.
Explanation: DNA replication requires both a template strand and a primer to
begin synthesis, while transcription only requires the template DNA strand.

4. As a sample of DNA is heated, it is said to melt. What is occurring?
Answer: B. As hydrogen bonds are broken, the two strands separate.
Explanation: DNA melting refers to the separation of the two strands when the
hydrogen bonds between base pairs are broken by heat.

5. The Shine-Delgarno sequence:
Answer: C. Base-pairs with 16s RNA.
Explanation: The Shine-Delgarno sequence is a conserved region in bacterial
mRNA that helps in the initiation of translation by base-pairing with the 16S rRNA
of the ribosome.

6. Ciprofloxacin is used as an antimicrobial agent because:
Answer: A. It inhibits the activity of bacterial topoisomerase II.
Explanation: Ciprofloxacin targets bacterial topoisomerase II (DNA gyrase),
preventing DNA supercoiling, which is essential for DNA replication.

, 7. The protein directly involved in separating the DNA strands during DNA
replication is called:
Answer: D. Helicase.
Explanation: Helicase is the enzyme responsible for unwinding the DNA double
helix during replication.

8. The 3'-5' exonuclease activity of DNA polymerase is critical for:
Answer: B. The fidelity of DNA synthesis.
Explanation: The exonuclease activity of DNA polymerase allows it to remove
incorrectly incorporated nucleotides, ensuring accurate DNA replication.

9. Which of the following statements concerning transcription is incorrect?
Answer: D. Rho-dependent termination involves a "hairpin" stabilized by G-C
base pairing near its base and a string of U's afterward.
Explanation: Rho-dependent termination does not rely on the formation of a
hairpin structure; instead, it involves the Rho protein recognizing the termination
signal and dissociating the RNA polymerase.

10. The filling of the gaps between the fragments of the lagging strand after
the removal of the multiple RNA primers is achieved by:
Answer: B. DNA polymerase I and DNA ligase.
Explanation: DNA polymerase I removes RNA primers and fills in the gaps with
DNA, and DNA ligase seals the nicks between Okazaki fragments.

11. The prokaryotic RNA polymerase holoenzyme has an α2 β β' σ subunit
structure. Which of the following statements is TRUE regarding this enzyme?
Answer: B. The σ subunit is only involved in initiation of transcription and
dissociates from the enzyme after RNA synthesis begins.
Explanation: The σ subunit is required for the initiation of transcription as it helps
RNA polymerase recognize promoter sequences, but it dissociates once elongation
begins.

In eukaryotes, DNA is present in a chromatin structure. Chromatin is made up of:

A. DNA that is highly compacted by compartmentalization in a series of vesicles.
B. DNA that is highly compacted by supercoiling.
C. DNA that is highly ordered by wrapping around histones.
D. DNA that is highly compacted by interaction with itself.
E. DNA that is tightly associated with the inner face of the nuclear membrane. -
ANSWER✔✔ C. DNA that is highly ordered by wrapping around histones.

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