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MCB 251 lon capa questions and answers with verified solutions 100% correct!!!
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MCB 251 lon capa questions andanswers with verified solutions 100%
correct!!!
Why is the aseptic technique necessary in microbiology? - ANSWER✔✔ Aseptic
technique is needed to avoid microorganisms from contaminating the experiment
You want to pipette and dispense 25 mcl of liquid. Which is the correct step
involved in this process? - ANSWER✔✔ Use the P-200 pipettor set to 25 mcl with
a tip
Which option is correct for balancing a centrifuge? - ANSWER✔✔ the amount
should be oppositely balanced. so 400 mcl facing each other
Define local dilution factor: - ANSWER✔✔ The local dilution factor is the dilution
factor relative to the previous n aliquot
What is a hypothesis - ANSWER✔✔ A proposed explanation of a phenomenon
Design an experiment to test if an unknown compound inhibits bacterial growth.
(provide hypothesis, positive and negative controls, identify a baseline for the
experiment, the experimental group, basic experimental design and how you
would determine the result of the experiment) - ANSWER✔✔ 1. hypothesis: An
unknown compound will inhibit bacterial growth.
2. Positive control: a plate with penicillin and bacteria (should have no growth, is
made to mimic the expected results)
3.Negative control: Plate with no penicillin and bacteria (lots of growth, this is to
mimic that your compound did not inhibit growth)
, 4. Baseline for experiment: This can be negative control to show that nothing had
died
5:Experimental groups: Need at least one concentration of the compound to see
if there was any change in growth
6.Experimental design: Adding compound to plate or bacteria, plating bacteria,
incubation of bacteria
7. Determine Results: Count colonies
What is the difference btw streak plates and spread plate technique? -
ANSWER✔✔ Streak plate technique: purpose if to obtain a pure culture and can
obtain a single colony that is isogenic to the previous colony. The loop should be
flamed before touching to bacterial stock. Loop also needs flamed btw streaks
from primary streak, and second streak.
Spread Plate technique:
The purpose is to obtain a spread out single bacteria colonies. Also, use dilutions
to obtain single colonies that are spread out. pipette bacterial sample in broth
onto plate and then use spreader (after it has been dipped in ethanol and flamed)
to evenly spread liquid across plate.
Calculate the global dilution factor, local dilution factor, and concentration for
test tubes A, B, and C. - ANSWER✔✔ A) Global: 5, Local: 5, Concentration: 3,125
B) Global: 625, Local: 5, Concentration: 25
C) Global: 3,125, Local: 5, Concentration: 5
Which of the following why Taq DNA polymerase is used for performing PCR? -
ANSWER✔✔ Taq DNA polymerase, isolated from thermophilic bacteria, can
withstand high temperature during PCR.
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