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Mcb 101 Lon Capa For Exam Ii Questions And Answers With Verified Solutions Already Passed !!!
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Mcb 101 Lon Capa For Exam IiQuestions And Answers With Verified
Solutions Already Passed !!!
Biochemical Tests and Enzymes/Metabolic Pathways:
1. INDOLE - Tryptophanase
2. METHYL RED - Mixed acid fermentation
3. H2S PRODUCTION - Cysteine desulfhydrase
4. VOGES-PROSKAUER - 2,3-butanediol fermentation
Reagents for Biochemical Tests:
1. INDOLE TEST - Kovac's Reagent
2. PHENYLALANINE DEAMINASE TEST - Ferric Chloride Solution
3. CATALASE TEST - 3% Hydrogen Peroxide
Positive Response Appearance:
1. Phenylalanine Deaminase Test - Green color on top of agar
2. Sugar Fermentation Test - Broth turns yellow
3. Indole Test - Reddish pink layer on top of broth
4. Hydrogen Sulfide Production Test - Black precipitate
Differentiating Streptococcus and Staphylococcus:
The best test to differentiate between Streptococcus and Staphylococcus is
the A. catalase test.
, (Staphylococcus is catalase positive, while Streptococcus is catalase
negative.)
Biochemical Test for Gas Production:
The Durham tube is used to check for gas production in A. Sugar
Fermentations.
Test for Identifying S. aureus or E. faecalis:
For a Gram positive coccus, the D. Catalase test should be performed to
differentiate S. aureus (catalase positive) from E. faecalis (catalase
negative).
First Day of Unknown Bacterial Identification:
On the first day, you will E. streak on L-agar, MacConkey agar, and
phenylethanol agar.
Descriptions of Possible Unknown Bacteria:
1. Enterococcus faecium - Gram + coccus, catalase -
2. Bacillus subtilis - Gram + rod, forms endospores
3. Escherichia coli - Gram - rod, fermentative metabolism
4. Staphylococcus aureus - Gram + coccus, catalase +
MATCH the names of the possible unknown bacteria with the description
Pseudomonas aeruginosa - ANSWER✔✔ Gram - rod, oxidative metabolism
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