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Microbiology LAB Exam #1 Questions and Answers 100% Pass
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Microbiology LAB Exam #1 Questions and Answers 100% Pass Microscope- The Condenser - focuses the light ging into the objective lens Microscope- The Iris Diaphragm - adjusts the amount of light going into the objective lens Microscope- Nosepiece - holds the objective lenses and rotates to chang...

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  • January 17, 2025
  • 18
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • microbiology lab exam 1 questions and answers 100

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  • Microbiology Lab
  • Microbiology Lab

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Microbiology LAB Exam #1 Questions
and Answers 100% Pass


Microscope- The Condenser - ✔✔focuses the light ging into the objective lens


Microscope- The Iris Diaphragm - ✔✔adjusts the amount of light going into the

objective lens


Microscope- Nosepiece - ✔✔holds the objective lenses and rotates to change from one

objective to another


Microscope- Coarse Objective Knob - ✔✔moves the stage larger distances to help get

the object in focus


Microscope- Fine Adjustment Knob - ✔✔moves the stage smaler increments to help get

the object in better focus


Microscope- Objective Lens - ✔✔magnifies the object. It is located near the object.


Microscope- Occular Lens - ✔✔magnifies the image of the object from the objective lens.

It is located near the eye.


Steps in using the microscope - ✔✔1) scanner power in place




Katelyn Whitman, All Rights Reserved © 2025 1

,2) place slide of specimen stage in place with stage clips


3) Using the course adjustment knob, raise the stage all the way up and then down

slowly until you can see image of specimen


4) Readjust with fine adjustment knob


Describe the purpose of the oil immersion lens - ✔✔Used when oil is added to the slide.

Oil must touch the object and objective lens to that the light does not refract futher


Parfocal - ✔✔Parfocal microscopes are able to move from one objective to the next and

still be in focus due to the length of the objective lenses


Resolution - ✔✔The ability to distinguish between two objects that are very close

together.


What is resolving power? - ✔✔The measurement of resolution


What are two ways to increase resolving power? - ✔✔1. By decreasing the wavelength

of light (using a blue filter) 2. Increasing the numerical aperature


What units are used to express resolving power? - ✔✔nm or nanometers


What is better, smaller or larger resolving power? - ✔✔Smaller is better


Light microscopes have a 200nm resolving power


Electron microscopes have a .2nm resolving power




Katelyn Whitman, All Rights Reserved © 2025 2

, What is chromatic aberration? - ✔✔The effect when many colors are seen toward the

edge of the microscope field of vision (like a prisim)


How are microscopes corrected for chromatic aberration? - ✔✔With a special

achromatic lens (corrected for red and blue light) or by using a blue filter. The lab has

blue filters in their microscopes which also increase resolution.


Describe the process of making a wet-mount slide - ✔✔1) Add the specimen to the slide


2) Touch the edge of the cover slip with one edge of drop of specimen.


3) Let the cover slip drop gradually to the slide, covering the drop of specimen.


Describe the purpose of making a wet-mount slide - ✔✔To be able to see living

organisms under the microscope in their natural state.


What is the advantage of using a wet-mount slide? - ✔✔Can see movement and

determine if microbes posses their own method of motility. Can also see the actual

shape and arrangement of microbes.


What is the dis-advantage of using a wet-mount slide? - ✔✔The movement is very fast,

microbes are transparent. Must use a "dark-field stop".


What is a producer? - ✔✔Are autotroph organisms that make their own food (through

photosynthesis).




Katelyn Whitman, All Rights Reserved © 2025 3

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