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Microbiology Lab - Exam 2 Questions and Answers 100% Pass
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Microbiology Lab - Exam 2 Questions and Answers 100% Pass medium - a mixture of nutritional substances on or in which microorganisms grow autotroph - nutritionally deficient mutants that have lost the ability to synthesize a particular enzyme many microorganisms in this group: - can carry out...

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  • January 17, 2025
  • 19
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • microbiology lab exam 2 questions and answers 10

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  • Microbiology Lab
  • Microbiology Lab

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Microbiology Lab - Exam 2 Questions
and Answers 100% Pass


medium - ✔✔a mixture of nutritional substances on or in which microorganisms grow


autotroph - ✔✔nutritionally deficient mutants that have lost the ability to synthesize a

particular enzyme




many microorganisms in this group:


- can carry out photosynthesis


- usually do not cause disease


- synthesize organic compounds to obtain energy


heterotroph - ✔✔an organism that uses compounds to produce biomolecules


autoclave - ✔✔an instrument for sterilization by means of moist heat under pressure


Autoclaving combines ____ and ____ to destroy high resistant bacteria. - ✔✔high temp

and high pressure




Katelyn Whitman, All Rights Reserved © 2025 1

,What are six basic nutritional requirements of every living thing? - ✔✔Water, carbon,

nitrogen, metallic elements, energy and growth factors such as vitamins


general purpose media - ✔✔Designed to grow a broad spectrum of microbes that do

not have special growth requirements


enriched media - ✔✔medium that contains special additives that could promote certain

metabolic activities. (ex. trypticase soy agar, blood agar, yeast extract broth)


- especially used for fastidious bacteria


selective media - ✔✔is a nutrient that inhibits the growth of a certain type of

microorganisms (ex. Gram + bacteria) while permitting the growth of a different type

(in this example, Gram - )


differential media - ✔✔medium which will differentiate between bacteria based on how

they change the medium or their appearance on the medium


EMB and HE are both ____ and ____. - ✔✔selective and differential


EMB and HE are selective for ____ and differential for ____. - ✔✔selective for gram-

negative bacteria and differential for lactose fermenters.


What do lactose fermenters and nonfermenters look like on each media? - ✔✔-

Organisms which quickly ferment lactose will give dark-green to black colonies on this

medium; these colonies have a metallic sheen (Ecoli)




Katelyn Whitman, All Rights Reserved © 2025 2

, - Organisms that are slow fermenters of lactose will give pink to maroon colonies on

this medium. Those organisms that cannot ferment lactose at all give white (colorless)

colonies on EMB agar (since nonfermenters of lactose are often high pathogenic, this is a

useful way to identify potential pathogens)


What is Shigella associated with? - ✔✔a genus associated with bacillary dysentery


HE and EMB are useful for distinguishing between _____. - ✔✔Enterobacteriaceae


What are Enterobacteriaceae? - ✔✔a group of short, Gram-negative, facultative

anaerobic rods that are found in the intestinal tract of various mammals, including

humans


PEA and MS agar are _____ for ____. - ✔✔selective for gram-positive bacteria


MS is _____ and _____ for _____. - ✔✔selective and differential for mannitol fermenters


What bacterial genus is Mannitol Salt (MS) agar used to distinguish? -

✔✔Staphylococcus -- high levels of salt in medium are tolerated by its cells


What do fermenters and non-fermenters look like on Mannitol Salt (MS) agar? - ✔✔-

fermentation of mannitol causes the medium to turn deep yellow in the area of bacterial

growth


- if mannitol not fermented, the color of the medium will remain pink




Katelyn Whitman, All Rights Reserved © 2025 3

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