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Microbiology Lab Exam 1 Questions and Answers 100% Pass
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Microbiology Lab Exam 1 Questions and Answers 100% Pass What is the purpose of a streak plate? - 1) To separate cells so that they grow to form isolated colonies 2) To confirm if a culture is a pure colony or mixed 3) To confirm is there is contamination in your culture What is a colony? - Are...

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  • January 17, 2025
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  • 2024/2025
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  • microbiology lab exam 1 questions and answers 100

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Microbiology Lab Exam 1 Questions
and Answers 100% Pass


What is the purpose of a streak plate? - ✔✔1) To separate cells so that they grow to form

isolated colonies 2) To confirm if a culture is a pure colony or mixed 3) To confirm is

there is contamination in your culture


What is a colony? - ✔✔Areas of growth that arise from a single cell.


How do colonies form? - ✔✔They are formed by spreading out cells. Cells are spread

and reproduce over and over again through bionary fission until visable mass of cells

appear.


What is a pure culture? - ✔✔Culture containing only one type of organism


What kind of media are colonies able to form on? - ✔✔agar slant, broth media, agar

plate


When is a agar slant used? Pros & cons - ✔✔1) Smaller amount of surface area prevents

the media from drying out too quickly 2) useful for medium term storage 3) viable for a

couple of months if stored properly




Katelyn Whitman, All Rights Reserved © 2025 1

,What are the 4 criteria for labeling a microbial culture? - ✔✔1) initials or name AND

group number


2) date


3) growth media type


4) culture name or source of inoculum


Describe bacillus (bacilli) morphology - ✔✔rod shaped cells, longer than wide, sausage

like, can use rod or bacillus


describe coccus morphology - ✔✔cells are ball shaped or spherical


Describe coccobacillus morphology - ✔✔elongated spheres- not quite spherical, but not

clearly a rod. Tends to occur in species which are normally rod-shaped during rapid

growth.


Describe spirillum morphology - ✔✔Helical, loose or tight coils. Look like wavy lines -

morphology has no arrangement


Describe filamentous morphology - ✔✔Long elongated cells. Look like a tangled mass

of branched filaments- no arrangement


Describe vibrio morphology - ✔✔A curved rod or comma shape


What does No arrangement mean? - ✔✔Single cells, ungrouped. Not all cells have an

arrangement



Katelyn Whitman, All Rights Reserved © 2025 2

, What is the cell arrangement Diplococci/Diplobacilli - ✔✔Pairs of cells. Diplococci are

pairs of cocci, diplobacilli are pairs of rods


What is the cell arrangement Streptococci/Streptobacilli? - ✔✔Chains of cells.

Streptococci are chains of cocci, streptobacilli are chains of rods


What is the call arrangement staphylococcus? - ✔✔Irregular clusters of cells- like

bunches of grapes.


What is the cell arrangement of tetrads? - ✔✔Sets of 4 regularly arranged cells


What is the cell arrangement of palisade? - ✔✔Bacilli (usually 2-4 cells) arranged side by

side. Columns or "V-forms"


What genus is an acid fast bacteria? - ✔✔Mycobacterium tuberculosis


What genus is an capsule former? - ✔✔Klebsiella pneumoniae


What is a simple stain? - ✔✔Involves only one dye to make cells more visable. Involves

methylene blue or crystal voilet.


What is a complex stain? - ✔✔Two or more stains. Allow the differentiation of cell types

or cell structures. (can be called differential stain)


What is a differential stain? - ✔✔Another name for complex stain. Allow us to see the

differences between different cell types or cell structures. Uses at least two different

stains.



Katelyn Whitman, All Rights Reserved © 2025 3

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