EXAM 3 MLS 4141 IMMUNOLOGY AND SEROLOGY CHPTER 11-16 QUESTIONS AND CORRECT DETAILED ANSWERS (VERIFIED ANSWERS) ||ALREADY GRADED A+||NEWEST VERSION
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Course
BMED 220
Institution
BMED 220
Quiz: Labled immunoassay
Ans: designed for Ags and Abs that ar esmall in size or prsent in very low
concentrations. Use a labled reactant ot monitor the amount of binding that has
taken place. Types: radioimmunoassay, enzyme immunoassay, fluorescent,
chemiluminescent. Bacterial antigens, hor...
1
EXAM 3 MLS 4141 IMMUNOLOGY AND
SEROLOGY CHPTER 11-16 QUESTIONS AND
CORRECT DETAILED ANSWERS (VERIFIED
ANSWERS) ||ALREADY GRADED A+||NEWEST
VERSION
Quiz: Labled immunoassay
Ans: designed for Ags and Abs that ar esmall in size or prsent in very low
concentrations. Use a labled reactant ot monitor the amount of binding that has
taken place. Types: radioimmunoassay, enzyme immunoassay, fluorescent,
chemiluminescent. Bacterial antigens, hormones, drugs, tumor, markers,
specific immunoglobulins
Quiz: Homogenous
Ans: No seperation step. Uses one Ab. Kit that comes with labled Ag and
corresponding Ab. Amount of labeled Ag/ab complex inversely related to
patient ag. Less sensitive than heterogenous assay
Quiz: Heterogenous
Ans: seperation step. Uses two different Abs. More sensitive than homogenous
assays
Ans: Uses one antibody, All reactnats added simultaneously. Labeled reagent
ag competes with unlabled patient Ag for limited number of reagent Abs. Ag/ab
complex inversely proportional to the amount of labeled ag/ab complex
Quiz: Noncompetitive assay
Ans: uses two different antibodies. 1st ab is passively absorbed to a solid phase.
patient AG captured by ab because it has bound to it. second ab with labeled is
added after washing. Directly proprtional relationship between labeled Abs and
amount of patient constitutent
Quiz: RIA
Ans: radioimmunoassay first immunoassay edeveloped. Radioative labels (125I
most popular). Isotopes emit gamma radiation which is detected by gamma
counter. Sensitive and precise. trace amount of analytes small in size. Label in
bound phase indirectly proportional to amount of patient Ag. Disadvantages:
radioactive waste and short shelf- life.
3
Ans: EIA- uses enzymes which react with suitable substrates to produce
breakdown products that may be chromegenic, fluoogenic or luminescent.
Alkaline phosphatase and horseradish peroxidase are most common. highest
turnover. High sensitivty and easy to detect.
Quiz: COMPETITIVE EIA
Ans: Enzyme labeled ag comptes wtih unlabled patient Ag fo rbinding site on
Abs. Used for measuring small Ags that are pure. high specificity. INverse
relationship to the test substancce. More patient Ag bound, less enzyme-labeled
ag can bind.
Quiz: Noncompetitive
Ans: measure Ab production to infectious agents that are difficlutl to isolate
and for autoantibody testing. HIgh sensivity and specificty, simplicity, low cost.
ELISA. easily adapted to POCT
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