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TTU Mbio Lab Exam 2 Questions with complete solution
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TTU Mbio Lab Exam 2 Questions with complete solution What genera (genus) does Stuart's Urea Broth test for? - correct answer - Proteus genus What pH indicator is used in Stuart's Urea Broth? - correct answer - phenol red What pH does it change for Urea positive colors and what color? ...

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  • February 19, 2025
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  • 2024/2025
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TTU Mbio Lab Exam 2 Questions with
complete solution
What genera (genus) does Stuart's Urea Broth test for? - correct answer ✔-
Proteus genus


What pH indicator is used in Stuart's Urea Broth? - correct answer ✔- phenol
red


What pH does it change for Urea positive colors and what color? - correct
answer ✔- phenol red turns from orange to pink at 8.4 pH
- positive = if it contains urea phenol red, it turns pink 8.4
Negative = stays orange


How does the Stuart's Urea broth work? - correct answer ✔to overcome the
buffer and raise the pH, the bacteria must produce ammonia


What enzyme is used to break down urea and into what molecule? - correct
answer ✔- urease = an enzyme that catalyzes the hydrolysis of ammonia and
carbon dioxide


Know that bacteria can utilize sugars differently, what test can be done to
check carbohydrate utilization of several sugars? - correct answer ✔-
carbohydrate fermentation
- common end products = pyruvic acid, H2 or CO2 gases, and alcohols
- if it utilizes gas, the Durham tube's liquid decreases
- we used glucose, lactose, sucrose and maltose broth for each bacteria

, What is the carbohydrate fermentation test made of? - correct answer
✔phenol red, peptone, carbohydrate


In carbohydrate fermentation, what pH indicator is used and what pH and
color does it change to positive result? - correct answer ✔- phenol red
-negative= pink = above 7.4 pH
- positive = yellow= less than 6.8 pH


What is the purpose of the inverted Durham tube? - correct answer ✔- to see
if gas was produced during metabolism


What is an antibiotic? - correct answer ✔- a type of chemical synthesized by
living organisms that has antimicrobial properties
- it can either kill the organism (bactericidial effect) or stop it from growing
(bacteriostatic effect)


How is an Antibiotic sensitivity test done? - correct answer ✔- by growing
pure colonies of the diagnosed infectious agent on agar plates and placing
antibiotic-soaked paper discs on top of the agar
- as the plates incubate - the antibiotics diffuse from the discs into the culture
medium
- the antibiotic becomes more diluted from the incubationand moves away
from the disc


What is the difference between bacteriostatic and bactericidal? - correct
answer ✔- kill the organism (bactericidial effect)
- stop it from growing (bacteriostatic effect)


What factors impact the zone of inhibition? - correct answer ✔- if the
antibiotic is effective or not

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