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Chamberlain College of Nursing:BIOS 242 Microbiology Midterm Exam Guide / BIOS242 Microbiology Midterm Exam Guide ( Version 2, New 2020)$18.49
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1. Pasteur - Final disproof spontaneous generation
2. Hook- Saw first microbes
3. Lister -Aseptic techniques during surgery
4. Semmelweis - Dr. had to wash hands in maternity ward
5. Schultze & Schwann- Chemical treatment of air stops ability to produce life
6. Koch - Developed postulates for disease microbe connection
7. Redi - Maggot-meat experiment
8. Leeuwenhoek - Made first microscope
Fundamental of cells:
Unicellular (Bacteria, Archaea, Protozoa, some fungi) and multicellular organisms (animals and
plants)
All cells (prokaryotes + eukaryotes) have in common:
Cell membrane
DNA that holds genetic information
Ribosomes for protein synthesis
Cytoplasm
Eukaryotes are more complex with DNA enclosed in nucleus and membrane enclosed
organelles
Bacterial shapes:
Cocci Rods. Vibrio Spirillum
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, Microbiology Midterm Study Guide
Spirochete Branching Filaments
The 5 Is:
Inoculation - Purposely moving something from 1 place to another. starts with specimen
collection; lesion, draw blood, bird droppings, etc.
- introducing a tiny sample into a medium to provide an env't where they multiply.
Incubation - To hear bacteria to make it grow (usually body temp). maintaining something at
the most favorable temperature for its development. 20 deg C & 40 deg. C02 may be required.
- promotes multiplication of microbes over period of hours.
- produces a culture
Isolation - Separate from each other
separation of a strain from a natural, mixed population of living microbes, spreading bacteria
apart as far as possible.
- isolated microbes takes the form of separate colonies on solid media or turbidity (free floating
cells) on broth.
Inspection - Doing tests on the bug
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