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Biochemistry TCA Cycle Lecture
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TCA cycleGlucose potential energy
aYycolyn f small E releate
TEAcycle little more E release
ETO MOST E released process Oxidative phosphorylation
Remember Atp formed a ADP phosphorylation 2ways
offidatrbh
phosphorylation phosphorylatio
substrate
level
summaryaerobic E production
pyruvate
coz Decarboxylation nun
Acetyl CoA
U
TCAcycle
o
E released oxidativeproceso
form coenzymes NADH
FADH2
e given coenzymes s Eto s 02 final e
acceptor acceptor
molecules
etransfer createproton gradientaccroromembrane
stored E drives ATPrynthetis
ETO oxidativephot ATPsynthesized
ufque
I glucote 2 glycolyn't
go zg Ayp 2TUA
INA ET O
Mon E created during oxidativephotphorylation
,24 e removed complete oxidation glucose
Glycolyn V 2ATP
4C 2e from 2 NADH's
1 pyruvate decarboxylation 2e X 2 pyruvate 4 e
TCA g gleATP x z benpyruvate
ETO 8C 202
t 41120 X 2
1Glucose 24e El 30 38 ATP
TCA enzyme8 Matrix mitochondria
EXCEPT tuccinatedehydrogenate
integralmembrane
protein linked to
innermitochondrial
membrane
, pyruvate source AcetylCoA
glycolytic pyruvate AcetylcoA 1A
cytoplasm oxidative carboxylation mitochon
before enteromitochon
4
Enzyme pyruvateDehydrogenasecomplex Pbo
Complex
pyruvate Coat NADt s Acetyl CoA COLT NADH Ht
RxnMech PDO
pyruvateDehydrogenase
3Enzymed Dihydrolipoamide acetyltransferase
Dihydrolipoamide dehydrogenase 1849
5 coenzyme8 Thiamine pyrophosphate
GASH
FAD
NAD
Lipoiona'd
mech pyruvate Decarboxylated
L CO2
20 unit transfer to lipoicacid
e
t ormfarfimnfEfyfaflipaa.cl
2Cunitlipacid WASH
Re oxidation Lipoivacid
allow mech repeat
NADHproduced enters ETO
(Garret and Grisham,
2016)
El EL E3
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