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Biochemistry Enzyme Kinetics and Specificity Lecture Notes
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Enzymes Kinetics specificityEnzymes proteinsspecialized to catalyzemetabolic ring
a substratespecificproteins
speedup rxns via j Activationenergy
Aid cells to exert kinetic control over thermodynamicpotentiality
s s
Hgafaarntity represents thermodynamic
state ofsystem
Accelerate El controlbiochemical rxn rates
speedhpts via regulatoryenzymeswhich
degradation transformgenerate promoteinhibit certain ring
ofnutrients metabolites
Enzymescontrol the rate of spontaneous rims DG0 because all
spontanicusrxns canoccur even withoutenzyme
Function without largeinputofenergy canfunction in lowtemperatures
DistinctFeatures
Catalyticpower rate catalyzed rxn
rate uncatalyzed rxn
specificity enzyme selective for tubstrate
chirality handedness
9specificity 9 yieldproduct
noformation of wastefulbyproducts
Regulation Rate metabolic run is maintained relative to
the cell's requirements
maintaincorrect Metabolites
Enzymes canbe switched onoff
up a dam regulated
fine levelofcontrol
Mildconditions mild temp pH
chemical catalystonlyfunction in extreme conditions
not feasible for physiological conditions
pH whichenzymefunctionsdetermineswherefoundin body
Enzymesalsofunction in extremeconditions bacteria
extremophiles
Highspecificity
productyields909o
enzymes don'tneedlots of rtn steps to achieveproduct
chemical catalysts need lots of rim steps
, (Garret and Grisham, 2016)
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