Biochemistry
Hofstra University
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The fundamentals of biochemistry notes provide a comprehensive overview of key principles and concepts essential for understanding the biochemistry.
Bioanalytical techniques and Amino Acids
Amino Acids and Mutation
Amino Acids and thermodynamics
Practice exams questions on chapter 1: Amino acids and Energy
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Invitro Synthesis of Luciferase protein using rabbit reticulocyte cell expression system
- Thesis • 13 pages • 2022
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This experiment explores the purification of T7 RNA polymerase and using this RNA polymerase to synthesize an RNA transcript followed by the in vitro synthesis of our protein of interest that is luciferase protein. The experiment helps to understand concepts of protein expression and purification through in-vitro transcription and translation. It is hypothesized 
 
that the purified T7 RNA polymerase will transcribe the linearized plasmid to luciferase mRNA transcript by in vitro transcription. ...
It is good review for exam. It includes, Bohr effect, hemoglobin vs myoglobin, ligand binding, hill plot.
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