Cryostat temperature - Study guides, Class notes & Summaries

Acetic acid - ANSWER-This fixative does not fix or destroy carbohydrates, and it does not fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative mixtures because of its ability to fix nuclei. Glutaraldehyde - ANSWER-Techniques using Schiff reagent, such as the periodic acid-Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results will be obtained. Glyoxal - ANSWER-This fixative is the smallest dialdehyde and is ...

T/F To be characterized as a profession, considerable specialized training must be required. - ANSWER-T Histology is the study of - ANSWER-tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. - ANSWER-T T/F Accessioning is giving a specimen a common number, one that is not unique to the patient. - ANSWER-f T/F The tasks performed by the histotechnologist require patience, mechanical ability, knowledge of biology,...

What thickness are paraffin sections routinely cut? - ANSWER-4-6 um Why should the temperature of the flotation bath be set specifically at 5-10 °C below the melting point of the paraffin wax? - ANSWER-water bath that is too hot may distort tissue morphology Warm enough to relieve compression Cool enough so the wax does not melt What thickness are paraffin sections cut for the Luxol Fast Blue procedure? - ANSWER-10 - 15 um Why are paraffin blocks put on ice prior to cutting? - ...

lysis or dissolving of cells by enzymatic action - ANSWER-Autolysis Breakdown of tissue by bacterial action - ANSWER-Putrefaction fixative classified as aldehyde - ANSWER-Formalin found in Bouin's fluid, is explosive and poisonous - ANSWER-Picric Acid good fixative for nuclei & gives great detail - ANSWER-Zenker's Fluid recommended for fixation of tissues for electron microscopy - ANSWER-Gluteraldehyde cytology fixative - ANSWER-95% ethanol fixative classified as oxidizing...

INTRODUCTION The future ATLAS experiment [1] at the CERN Large Hadron Collider (LHC) will include in the central (‘barrel’) region a calorimeter system composed of two separate units: a liquid argon (LAr) electromagnetic (EM) calorimeter with hermetic accordion geometry, and a scintillating-tile hadronic calorimeter using iron as the absorber, in which the tiles are placed perpendicular to the colliding beams. This system must be capable of identifying electrons, photons, and jets and ...

Explain the steps involved in tissue processing - ANSWER-1. Surgery 2. Fixation 3. Grossing 4. Fixation 5. Processing 6. Embedding 7. Staining 8. Cover-slipping Discuss the process of fixation - ANSWER-Stabilization of proteins to prevent autolysis and putrefaction. -Maintain proper relationship between cells and extracellular material (keep as "life-life" as possible) -15-20 times volume fixative of tissue -10% NBF most common fixative Describe the goal of tissue proces...

Oxytocin and antidiuretic hormone are two of the hormones secreted by the - ANSWER-Pituitary The primary antibody must be diluted 1:100 for an immunohistochemical procedure. How many micro liters of the primary antibody would be required to prepare a total volume of 2mL? - ANSWER-240 How many grams of NaOH (MW=40) are required to prepare a liter of 1N NaOH? - ANSWER-40 A one normal solution is equivalent to: - ANSWER-1 gram-equivalent weight of solute per 1L of solution (g/L) The num...

CPC Practice Exam Questions Surgical removal - ANS The suffix -ectomy means Magnetic Resonance Imaging - ANS MRI stands for The removal of the fallopian tubes and ovaries - ANS The term "Salpingo-Oophorectomy" refers to Freezing - ANS Cryopreservation is a means of preserving something through Paracentesis - ANS Which of the following describes the removal of fluid from a body cavity Gastrotomy - ANS If a surgeon cuts into a patient's stomach he has perfo...