Double stranded breaks Study guides, Class notes & Summaries
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![CMMB 403 Exam Prep New Questions and Answers 2024 |Updated & Verified](/docpics/4341839/65b40766c2b23_4341839_121_171.jpeg)
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CMMB 403 Exam Prep New Questions and Answers 2024 |Updated & Verified
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what does CRISPR use in order to generate changes in genome? is CRISPR forward or 
reverse genetics? - nuclease 
- 20 nucleotide RNA sequence to target specific sites in the genome 
- reverse genetics 
what is the role of Cas9 in CRISPR endonuclease that catalyzes double stranded breaks 
what are the two repair paths cells can use to repair double stranded breaks from CRISPR- 
Cas9 1. non-homologous end joining (NHEJ) 
- naturally try to repair 
- imperfect, creates indels causing frameshift mut...
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CMMB 403 final exam
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CMMB 403 final exam 
what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse 
genetics? - Answer ️️ -- nuclease 
- 20 nucleotide RNA sequence to target specific sites in the genome 
- reverse genetics 
what is the role of Cas9 in CRISPR - Answer ️️ -endonuclease that catalyzes double stranded 
breaks 
what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - 
Answer ️️ -1. non-homologous end joining (NHEJ) 
- nat...
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CMMB 403 final exam Question and answers already passed
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CMMB 403 final exam Question and answers already passed CMMB 403 final exam 
 
 
what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - correct answer - nuclease 
- 20 nucleotide RNA sequence to target specific sites in the genome 
- reverse genetics 
 
what is the role of Cas9 in CRISPR - correct answer endonuclease that catalyzes double stranded breaks 
 
what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Ca...
![AAB Molecular Diagnostics questions and answers(latest update)](/docpics/5355252/66492e48e6fd1_5355252_121_171.jpeg)
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AAB Molecular Diagnostics questions and answers(latest update)
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Nucleic Acid 
grows by attachment of 5' phosphate group of the incoming nucleotide to the 3'hydroxyl group of the last nucleotide 
 
 
 
double stranded DNA 
most energetically favorable state for DNA 
 
 
 
Purines 
double-ring structure Adenine (A) + Guanine (G) 
 
 
 
Pyrimidine 
single-ring structure Uracil (U)+ Cytosine 
 
 
 
Messenger RNA (mRNA) 
-template for all protein synthesis 
-encode all information necessary to produce proteins 
 
 
 
Heterogeneous nuclear RNA 
primary product o...
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AAB Molecular Diagnostics- Nicole's questions and answers
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Nucleic Acid 
grows by attachment of 5' phosphate group of the incoming nucleotide to the 3'hydroxyl group of the last nucleotide 
 
 
 
double stranded DNA 
most energetically favorable state for DNA 
 
 
 
Purines 
double-ring structure Adenine (A) + Guanine (G) 
 
 
 
Pyrimidine 
single-ring structure Uracil (U)+ Cytosine 
 
 
 
Messenger RNA (mRNA) 
-template for all protein synthesis 
-encode all information necessary to produce proteins 
 
 
 
Heterogeneous nuclear RNA 
primary product o...
![Biology 115 Exam 3 WVU Latest Update Graded A+](/docpics/5341113/6647c2c886505_5341113_121_171.jpeg)
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Biology 115 Exam 3 WVU Latest Update Graded A+
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Biology 115 Exam 3 WVU Latest Update 
 
Graded A+ 
 
Meiosis DNA replication prior to cell division 
 
Sister Chromatids are two copies of the same DNA(have the exact same sequence) 
 
Helicase Breaks H-bonds to open the replication bubble 
 
Topoisomerase Breaks a single covalent bond ahead of the replication bubble 
 
Single-stranded DNA binding proteins Prevents the re-annealing of a single stranded DNA 
 
DNA Polymerase III recognized double stranded DNA/RNA hybrid and synthesized DNA 
from ...
![CMMB 403 final exam](/docpics/4335332/65b3670437a99_4335332_121_171.jpeg)
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CMMB 403 final exam
- Exam (elaborations) • 28 pages • 2024
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CMMB 403 final exam 
what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse 
genetics? - Answer ️️ -- nuclease 
- 20 nucleotide RNA sequence to target specific sites in the genome 
- reverse genetics 
what is the role of Cas9 in CRISPR - Answer ️️ -endonuclease that catalyzes double stranded 
breaks 
what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - 
Answer ️️ -1. non-homologous end joining (NHEJ) 
- nat...
![CMMB 403 Final Exam with complete solution](/docpics/4343250/65b42af67af41_4343250_121_171.jpeg)
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CMMB 403 Final Exam with complete solution
- Exam (elaborations) • 28 pages • 2024
- Available in package deal
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CMMB 403 Final Exam with complete 
solution 
what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse 
genetics? - answer- nuclease 
- 20 nucleotide RNA sequence to target specific sites in the genome 
- reverse genetics 
what is the role of Cas9 in CRISPR - answerendonuclease that catalyzes double stranded 
breaks 
what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - 
answer1. non-homologous end joining (NHEJ) 
- natura...
![CMMB 403 Final Exam Actual Questions and Answers with complete solution](/docpics/4343298/65b42c55ae94b_4343298_121_171.jpeg)
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CMMB 403 Final Exam Actual Questions and Answers with complete solution
- Exam (elaborations) • 28 pages • 2024
- Available in package deal
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- $12.49
- + learn more
CMMB 403 Final Exam Actual Questions 
and Answers with complete solution 
what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse 
genetics? - answer- nuclease 
- 20 nucleotide RNA sequence to target specific sites in the genome 
- reverse genetics 
what is the role of Cas9 in CRISPR - answerendonuclease that catalyzes double stranded 
breaks 
what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - 
answer1. non-homologous...
![CMMB 403 Final Exam Actual Questions and Answers with complete solution](/docpics/4393579/65bba9df0319c_4393579_121_171.jpeg)
-
CMMB 403 Final Exam Actual Questions and Answers with complete solution
- Exam (elaborations) • 28 pages • 2024
- Available in package deal
-
- $12.49
- + learn more
CMMB 403 Final Exam Actual Questions 
and Answers with complete solution 
what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse 
genetics? - answer- nuclease 
- 20 nucleotide RNA sequence to target specific sites in the genome 
- reverse genetics 
what is the role of Cas9 in CRISPR - answerendonuclease that catalyzes double stranded 
breaks 
what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - 
answer1. non-homologous...
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