Aab molecular diagnostics - Study guides, Class notes & Summaries

AAB Molecular Diagnostics- Nicole's Flashcards Question and Answer 100% Correct Nucleic Acid - grows by attachment of 5' phosphate group of the incoming nucleotide to the 3'hydroxyl group of the last nucleotide double stranded DNA - most energetically favorable state for DNA Purines - double-ring structure Adenine (A) + Guanine (G) Pyrimidine - single-ring structure Uracil (U)+ Cytosine Messenger RNA (mRNA) - -template for all protein synthesis -encode all information necessary to pr...

AAB Molecular Diagnostics DNA & RNA Revised Exam [100% PASS] A double helical structure first described by Watson and Crick? *Ans* DNA (deoxyribonucleic acid) What are the 4 nitrogen bases that make up the majority of DNA? *Ans* Adenine, cytosine, guanine, and thymine. Consists of a five carbon sugar with the first carbon covalently joined to a nitrogen base and the fifth carbon joined to a triphosphate moiety? *Ans* Nucleotides A nitrogen base bound to an unphosphorylated sugar? *Ans...

AAB Molecular Diagnostics DNA & RNA Revised Exam [100% PASS] A double helical structure first described by Watson and Crick? *Ans* DNA (deoxyribonucleic acid) What are the 4 nitrogen bases that make up the majority of DNA? *Ans* Adenine, cytosine, guanine, and thymine. Consists of a five carbon sugar with the first carbon covalently joined to a nitrogen base and the fifth carbon joined to a triphosphate moiety? *Ans* Nucleotides A nitrogen base bound to an unphosphorylated sugar? *Ans...

AAB MT Molecular Diagnostics Enzymes VERIFIED EXAM [100% PASS] /verified/ EcoR1, HindIII, BamHI, NotI, and PacI are all examples of which class of restriction enzymes? *Ans* Class II This class of restriction enzymes account for at least 90% of enzymes used in molecular biology? *Ans* Class IIP (Palindromic specificity) How many base pairs are normally recognized by Class II restriction enzymes? *Ans* 4-8 base pairs (6-8 allows for larger fragments) This restriction enzyme class typic...

This DNA amplification method amplifies the nucleic acid used as a probe? - Answer- Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - Answer- DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - Answer- Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - Answer- Better specificity How does Ligase Chain Reaction differ from Polymeras...

AAB Molecular Diagnostics Testing QUESTIONS WITH COMPLETE SOLUTIONS

This DNA amplification method amplifies the nucleic acid used as a probe? - ANSWER Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - ANSWER DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - ANSWER Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - ANSWER Better specificity How does Ligase Chain Reaction differ from Polymerase Chain ...

AAB Molecular Diagnostics Testing Question and Answer 100% Correct This DNA amplification method amplifies the nucleic acid used as a probe? - Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - Better specificity How does Ligase Cha...

AAB MT Molecular Diagnostics Enzymes Question and Answer 100% Correct EcoR1, HindIII, BamHI, NotI, and PacI are all examples of which class of restriction enzymes? - Class II This class of restriction enzymes account for at least 90% of enzymes used in molecular biology? - Class IIP (Palindromic specificity) How many base pairs are normally recognized by Class II restriction enzymes? - 4-8 base pairs (6-8 allows for larger fragments) This restriction enzyme class typically recognizes mod...

AAB Molecular Diagnostics DNA & RNA Question and Answer 100% Correct A double helical structure first described by Watson and Crick? - DNA (deoxyribonucleic acid) What are the 4 nitrogen bases that make up the majority of DNA? - Adenine, cytosine, guanine, and thymine. Consists of a five carbon sugar with the first carbon covalently joined to a nitrogen base and the fifth carbon joined to a triphosphate moiety? - Nucleotides A nitrogen base bound to an unphosphorylated sugar? - Nucleosi...