Light microscopy Study guides, Class notes & Summaries

MIP 616 Exam 1 Questions and Answers All Correct which detergent is used to disrupt protein structure for electrophoretic analysis on polyacrylamide gels - Answer-SDS what is the best technique to localize catalase in peroxisomes - Answer-A catalase monoclonal antibody and transmission electron microscopy the purpose of treating tissue from an embryonic chick with trypsin and EDTA when generating a primary cell culture is to - Answer-digest protein-protein interactions that hold cells to...

Light microscopy CORRECT ANSWER Limited ability to visualize bacterial components or distinguish between bacteria without doing something extra Electron microscopy CORRECT ANSWER Can view exterior and internal structures, smallest specimens Phase-contrast microscopy CORRECT ANSWER Refracts the light that goes through the specimen; can see detail, live cells Fluorescence microscopy CORRECT ANSWER Use of fluorochromes that emit light of specific wavelengths when excited by light of a diff...

Light microscopy CORRECT ANSWER - Uses visible light to examine microscopic-sized objects that cannot be seen by the naked eye. - Allows finer detail than the naked eye. Electron microscopy CORRECT ANSWER - Visualize objects much smaller than those resolved optically. - Use accelerated electrons instead of light photons. - Samples are fixed with chemicals to cross-link molecules to preserve their structure. Fluorescent stains CORRECT ANSWER - Used with light microscopy. - Fluorescence ...

AMT MT Study Guide Questions and Answers Already Passed The three major phases of laboratory testing that a QA program should evaluate include a. mean, standard deviation, and coefficient of variation. b. pre-op, operative, and post-op. c. pre-analytical, analytical, and post-analytical. d. outpatient, inpatient, and non-patient. c. pre-analytical, analytical, and post-analytical. The process by which test results achieve the same high levels of accuracy and precision that can be reproduced...

Chapter 1: microbial cell structure and function CORRECT ANSWER Microscopy: compound microscope CORRECT ANSWER •Compound microscope -- Two sets of lenses form the image (Figure 1.15a) •Objective lens and ocular lens Microscopy: magnification CORRECT ANSWER •Total magnification = objective magnification ocular magnification • •Magnification: the ability to make an object larger Microscopy: resolution CORRECT ANSWER •Resolution: the ability to distinguish two adjacent obj...

Resolution Correct Answer-small dist b/t 2 pts can be distinguished; D = .61λ/Nsinα, want lower D; Light microscope 200nM Bright field microscopy Correct Answer-normal light type Phase-contrast Correct Answer-altered phase interference of unobstructed light; looks like cells are surrounded by light DIC Correct Answer-3D looking cells Confocal Correct Answer-3D imaging with fluorescence, progessively capture full surface in focus by removing out of focus; normal point-scanning vs....

Prepare: History of Microbiology • Does not have membrane bound cellular organelles and has cell wall made up of peptidoglycan: • According to Binomial system the scientific name of any living organism has (select all that apply): • He was the first person to use swan neck flask to disapprove the theory of spontaneous generation: • Father of Microbiology who called microbes animalcules: • Which field of microbiology monitors and controls the spread of diseases in communities? S...

What are the different types of microscopy and staining and when are they used? CORRECT ANSWER Types of microscopy include: light microscopy- used primarily to get an idea of the form of the bacteria, used in combination with staining to get an idea of Gram positive and Gram negative protein. Green fluorescent is used to observe cell occurrences like binary fission and movement of proteins. Electron microscopy is very detailed at getting an idea of what is occurring inside and outside of the ce...

Bio principles unit 2 brooks questions and answers graded A+ all organisms are made of cells and come from cells Cell theory When the cell size (INCREASES or DECREASES), the membrane gets smaller compared to cell volume. increases the study of cells Cytology "how can cork float" Took sections, looked under microscope and found that they had tiny chambers (aka cells) Robert Hooke Part of electromagnetic spectrum via sun, delivering energy that can be recorded as waveleng...

Which term is used to refer to a wave's rate of vibration? Ans- B. frequency On the figure, which letter represents a refracted ray of light? Ans- C (BUT D on test) On the electromagnetic spectrum, which type of wave has the shortest wavelength? Ans- A. cosmic ray Dyes are often used during microscopy to increase which of the following? Ans- A. contrast Which of the following was the first to use the term "cells"? Ans- A. Robert Hooke Which of the following is the typical magnific...