Bios 390 week 1 lab 1 - Study guides, Class notes & Summaries

BIOS390 – Molecular Diagnostics Week 1 (TCO 1) The basic principles of genetics contributed by Gregor Johann Mendel are: 1. The principle of segregation 2. The principle of independent assortment (TCO 1) The Augustinian monk, Gregor Johann Mendel, crossed yellow-seeded and green-seeded pea plants and then allowed the offspring to self-pollinate to produce an F2 generation. The results were as follows: 6018 yellow and 2002 green (8020 total). The allele for green seeds has what relati...

BIOS 390 WEEK 6: LAB: isolate Plasmid DNABIOS 390 BIOS . BIOS 390 WEEK 6: LAB: isolate Plasmid DNA Once a plasmid has been introduced into competent bacterial cells and the cells have grown into colonies on a medium selective for cells containing the plasmid, the next step is to perform a miniprep of the plasmid DNA in preparation for DNA sequencing. The three steps are: 1) growing cells in liquid culture; 2) purifying the plasmid DNA from the culture; and 3) performing a restriction diges...

BIOS 390 WEEK 5: LAB: transcription in prokaryotes Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to make an RNA molecule. Before transcription can take place, the DNA double helix must unwind near the gene that is getting transcribed. The region of opened-up DNA is called a transcription bubble. Transcription uses one of the two exposed DNA strands as a template; this strand is called the template strand. The RNA product is complementary to the ...

BIOS 390 WEEK 5: LAB: transcription in prokaryotes Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to make an RNA molecule. Before transcription can take place, the DNA double helix must unwind near the gene that is getting transcribed. The region of opened-up DNA is called a transcription bubble. Transcription uses one of the two exposed DNA strands as a template; this strand is called the template strand. The RNA product is complementary to the ...