Steps of pcr - Study guides, Class notes & Summaries

Males and Females are equally affected - Autosomal The process of copying DNA in the lab. - Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. - What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating - What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) - What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers stick ...

The pathophysiology of Glazmann thrombasthenia is: A. Deficiency of dense granules B. Deficiency of glycoprotein Ib/IX receptor C. Deficiency of glycoprotein IIb/IIIa receptor D. deficiency of microtubules - C. Deficiency of glycoprotein IIb/IIIa receptor Chagas disease is caused by: A. Trypanosoma cruzi B. Trypanosoma palladium C. Trypanosoma brucei D. None of the above - A. Trypanosoma cruzi What would a patient with a microcyctic/hypochromic anemia most likely have noted on their CB...

BIO 111 Lab Final – TAMU (2023/2024) (Complete and Accurate) coding region comprised of genes that encode proteins (instructions that determine traits) and makes up 1-2% of human DNA. non-coding gene makes up 98-99% of human DNA, contains STRs and is responsible for regulatory functions such as gene transcription short tandem repeats (STRs) unique repeating patterns of the same nucleotide sequence. They can be used to differentiate one person from another. DNA Profile specific pattern of DN...

What is the goal of PCR? Correct Answer-To copy and amplify a DNA sequence. What are the requirements of PCR? Correct Answer-Template, primers, deoxyribosenucelotides, DNA polymerase, buffer What is are the general steps of PCR? Correct Answer- What is the purpose of Microarray/chip based hybridization? How do we accomplish this? Correct Answer-We want to get a GLOBAL profile of gene expression. We do this by determining the number of genes expressed in a cell and with how much abundanc...

After experiencing extreme fatigue and polyuria, a patient's basic metabolic panel is analyzed in the laboratory. The result of the glucose is too high for the instrument to read. The laboratorian performs a dilution using 0.25 mL of patient sample to 750 microliters of diluent. The result now reads 325 mg/dL. How should the technologist report this patient's glucose result? A. 325 mg/dL B. 1300 mg/dL C. 975 mg/dL D. 1625 mg/dL - B; The correct answer for this question is 1300 mg/dL. The ...

Concerning antigen-antibody reactions, the prozone effect causes which of the following results? A. False positive B. False negative C. No reaction D. Mixed field reaction - correct answer B. False negative The following characteristics are applicable to Interferon-Gamma Release Assays (IGRAs) except: A. Can be used in patients with active tuberculosis disease symptoms B. Preferable for patients who have received a bacillus Calmette-Guérin (BCG) vaccination C. Can be used instea...

Males and Females are equally affected - Autosomal The process of copying DNA in the lab. - Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. - What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating - What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) - What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers stick ...

How does BPA cause cancer? - it disrupts meiosis and mitosis causing breast and prostate cancer. 3 and 7 plastics may contain BPA What is a genetic fingerprint? how do you make one? - -Genetic Fingerprint: DNA uses the differences in base sequences to identify individuals. -DNA fingerprinting involves 3 tools: 1. PCR to get large amounts of DNA 2. Restriction digest with blunt ends to fragment the DNA with precision 3. Gel electrophoresis to separate and visualize the DNA fragments ...

BMSC 210 Midterm 2 | 100% Correct Answers | Verified | Latest 2024 Version What is genetic engineering? - using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - expr...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Chapter 13 - DNA Purification, Agarose Gel Electrophoresis, and DNA Visualization - answer The genetic information of all living organisms is contained within the sequence of ____ ____ = Examples include: purines and pyrimidines = For most organisms, the genetic information is contained in nucleic acids of the deoxyribonucleic acid (DNA), or ribonucleic acid (RNA) in certain viruses - answernucleic acids ______ is a complete s...