Aldehyde fixatives Study guides, Class notes & Summaries

An example of an additive is one that contains: A. Picric acid B. Acetic acid C. Ethyl alcohol D. Acetone - correct answer Picric Acid When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will show: A. Better ultrastructural preservation B. Decreased immunoreactivity C. Increased enzyme activity D. Superior nuclear detail - correct answer Superior nuclear detail Micr...

CSMLS HISTOLOGY REVIEW EXAM QUESTIONS WITH CORRECT ANSWERS 100% 2024

T/F To be characterized as a profession, considerable specialized training must be required. - ANSWER-T Histology is the study of - ANSWER-tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. - ANSWER-T T/F Accessioning is giving a specimen a common number, one that is not unique to the patient. - ANSWER-f T/F The tasks performed by the histotechnologist require patience, mechanical ability, knowledge of biology,...

Oxytocin and antidiuretic hormone are two of the hormones secreted by the - ANSWER-Pituitary The primary antibody must be diluted 1:100 for an immunohistochemical procedure. How many micro liters of the primary antibody would be required to prepare a total volume of 2mL? - ANSWER-240 How many grams of NaOH (MW=40) are required to prepare a liter of 1N NaOH? - ANSWER-40 A one normal solution is equivalent to: - ANSWER-1 gram-equivalent weight of solute per 1L of solution (g/L) The num...

lysis or dissolving of cells by enzymatic action - ANSWER-Autolysis Breakdown of tissue by bacterial action - ANSWER-Putrefaction fixative classified as aldehyde - ANSWER-Formalin found in Bouin's fluid, is explosive and poisonous - ANSWER-Picric Acid good fixative for nuclei & gives great detail - ANSWER-Zenker's Fluid recommended for fixation of tissues for electron microscopy - ANSWER-Gluteraldehyde cytology fixative - ANSWER-95% ethanol fixative classified as oxidizing...

Resolution - ANSWER-Ability of a microscope to distinguish two objects as separate What do you call a measurement of 10^-6 m? - ANSWER-Micrometer, or micron Light Microscope - ANSWER-microscope that uses a beam of light passing through one or more lenses to magnify an object What resolving power does a light microscope have? - ANSWER-0.25 micrometers Types of Light microscopes (4) - ANSWER-- Simple (Dissecting) - Compound (What we use in lab to view slides regularly) - Dark Field (...

What thickness are paraffin sections routinely cut? - ANSWER-4-6 um Why should the temperature of the flotation bath be set specifically at 5-10 °C below the melting point of the paraffin wax? - ANSWER-water bath that is too hot may distort tissue morphology Warm enough to relieve compression Cool enough so the wax does not melt What thickness are paraffin sections cut for the Luxol Fast Blue procedure? - ANSWER-10 - 15 um Why are paraffin blocks put on ice prior to cutting? - ...

BOC Fixation(Questions and Answers 100% correct. An example of an additive fixative is one that contains: A. Picric Acid B. Acetic Acid C. Ethyl Alcohol D. Acetone Picric Acid When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will show: A. Better ultrastructural preservation B. Decreased immunoreactivity C. Increased enzyme activity D. Superior nuclear detail Superior nuclear detail 00:21 01:08 Microscopic examination of an H&E stained sect...