Mcb 2050 midterm - Study guides, Class notes & Summaries

MCB 2050 Molecular Biology of the Cell - W21 complete midterm exam notes (get well prepared) University of Guelph

MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam PART A – Questions from tutorial Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a particular gene in a whole mounted embryo? a) Western blotting b) Northern blotting c) Southern blotting d) Immunoprecipitation e) RNA in situ hybridization Question 2. Why is Taq DNA polymerase ...

MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant selectable marker gene c. An origin of replication d. A Taq polymerase binding site 2. Methylation of DNA sequence is used how by bacteria? a. Methylation of DNA can inhibit cell division b. Methyla...

MCB 2050 Midterm Exam Questions and Answers 100% Solved 1) What are restriction endonucleases? 2) What do they do (or cut) specificically? 3) What happens to the ends of DNA cut by restriction enzymes? - 1) enzymes that cut at specific sequences (restriction sites) within DNA molecules 2) they break phosphodiester bonds that link nucleotides together 3) have single-stranded overhangs; ends are cohesive (complementary, sticky)- they can reanneal 1) Who discovered restriction enzymes? 2...

MCB*2050 Molecular Biology of the Cell W21 Midterm Examination, SAMPLE EXAM with Prof. K. Yankulov University of Guelph PART A (Choose only one and the best answer among the choices given.) 1. What happens when you use a mixture of four fluorescent 2′,3′-dideoxyribonucleoside triphosphates in DNA sequencing protocols? a. Multiple DNA chains that terminate at the 3’ ends at all possible nucleotide positions are produced b. When a 2′,3′-dideoxyribonucleoside triphosphate is added t...

MCB*2050 Midterm Prep Exam Questions and Answers 100% Solved RNA polymerase I - - in nucleus of eukaryotic cells - transcribes Pre-rRNA (28S, 18S, 5.8S rRNAs) - make ribosome components and synthesize proteins RNA polymerase II - - in nucleus of eukaryotic cells - transcribes mRNA, snRNAs, siRNAs, and miRNAs - encodes protein, RNA splicing, chromatin-mediated repression, and translation cotrol mRNA - - messenger RNA - transcribed by RNA polymerase II - encodes protein snRNAs - - tra...