Scsu bio120 lab Study guides, Class notes & Summaries
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SCSU BIO120 Lab - Exam I Questions And Answers
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*Negative Staining* Procedure - 1. Add a droplet of water 
2. Using an aseptic technique, place a loopful of the microorganism from the culture in the droplet and 
mix 
3. Place another slide at a 45° angle in a drop of nigrosin at the edge of the slide with mix 
4. Push or pull the slide over the the mix to form thin layer over. Leave to air dry 
Bacillus 
(Bacilli pl.) - *Rod* shaped bacteria 
Because of a snowstorm, your regular laboratory session was canceled and the Gram staining procedure...
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SCSU BIO120 Lab Exam I Questions with 100% Correct Solution
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Procedure to make a *Simple Stain* - Answer 1. Prepare a bacterial smear on a slide, 
making sure to heat fix it 
2. Then drown the smear with a stain, making sure to let it rest in the stain for the 
appropriate time (carbol fuchsin = 15 to 30 seconds, crystal violet = 20 to 60 seconds, 
and methylene blue = 1 to 2 minutes) 
3. Gently wash the smear with tap water to remove excess stain 
4. Using bibulous paper, *blot* the slide dry 
5. Examine slide under oil emersion
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SCSU BIO120 Lab Exam I Practice Questions and Answers (100% Pass)
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SCSU BIO120 Lab Exam I Practice 
Questions and Answers (100% Pass)
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