Southern blotting - Study guides, Class notes & Summaries
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MCB 2050 Molecular Biology of the Cell - W21 upcoming Midterm exam preparation material – Dr. Yankulov (via Respondus Lockdown Browser and Webcam) University of Guelph
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MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam 
 
PART A – Questions from tutorial 
Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a particular gene in a whole mounted embryo? 
a)	Western blotting 
b)	Northern blotting 
c)	Southern blotting 
d)	Immunoprecipitation 
e)	RNA in situ hybridization 
Question 2. Why is Taq DNA polymerase ...
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Genetics Final Exam|| 150 Questions & Answers|| 100% Verified
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What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - ANSWER ACETYLATION 
 
Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - ANSWER the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message 
 
Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - ANSWER Hybridizes filter-bound DNA wi...
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MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph
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MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph 
 
 
 
 
 
 
PART A 
 
1.	Which of the following is NOT an essential component of a cloning vector? 
a.	A unique restriction endonuclease cleavage 
b.	A dominant selectable marker gene 
c.	An origin of replication 
d.	A Taq polymerase binding site 
 
2.	Methylation of DNA sequence is used how by bacteria? 
a.	Methylation of DNA can inhibit cell division 
b.	Methyla...
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Genetics Final Exam 2023
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What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION 
 
Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message 
 
Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...
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Gel Electrophoresis Questions and Answers Rated A+
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Gel Electrophoresis Questions and 
 
Answers Rated A+ 
 
What role does gel electrophoresis play in gene therapy research? 
Gel electrophoresis is used to analyze the delivery and integration of therapeutic genes into 
target cells, helping assess the effectiveness of gene therapy approaches. 
 
How can gel electrophoresis help identify pathogens in a clinical setting? 
By amplifying specific genetic markers of pathogens and separating them using gel 
electrophoresis, researchers can quickly ide...
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Biotechnology |Recombinant DNA Questions and Answers
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A molecular technique in which DNA sequences between two oligonucleotide 
primers can be amplified is known as 
A. southern blotting 
B. northern blotting 
C. polymerase chain reaction 
D. DNA replication
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BCHM 270- All Questions100% Solved with Verified Solutions
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They can also be designed to only bind to one version of an allele - allele specific 
oligonucleotides (ASO). This can be used with PCR to report on whether samples 
contain certain mutations or polymorphisms. 
Southern Blotting - Answer Transfer of macromolecules onto a solid-phase membranous 
support such as nylon or nitrocellulose. This is done after gel electrophoresis and before 
hybridization in order to visualize results of these tests. 
Southern blotting refers to DNA blotting
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AAMC FL 5 B/BC QUESTIONS AND ANSWERS
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Adaptive immunity - Answer- specific 
 
Two types of adaptive immunity - Answer- Humoral immunity- B cells regulating antibodies 
Cell Mediated Immunity- T cells regulating 
 
innate immunity - Answer- non-specific 
 
phosphodiester linkage - Answer- covalent bonds that join adjacent nucleotides between the -OH group of the 3' carbon of one nucleotide and the phosphate on the 5' carbon of the next 
 
disulfide bonds - Answer- 2 cysteine residues can covalently bond to form disulfide bonds whic...
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Craniofacial Biology, Exam 1 with Practice Questions and Answers
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Craniofacial Biology, Exam 1 with Practice Questions and Answers 
 
Name two specific purposes for cloning foreign DNA into plasmid vectors ANSWER protein drug production (like insulin) and gene therapy 
 
Briefly describe the cloning process ANSWER -restriction endonuclease digests and inserts the DNA into a vector with ligation 
- introduce newly ligated DNA into host cell (like E coli) 
-identify bacteria that have taken up the proper + vector 
 
Where to RE come from and what do Restriction ...
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BCHM102 Midterm Exam Practice Questions and Answers
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BCHM102 Midterm Exam Practice 
Questions and Answers 
aminoacyl tRNA synthetase requires ______ energy - Correct Answer️️ -ATP 
translocation requires ________ energy - Correct Answer️️ -GTP 
two methods of translation termination - Correct Answer️️ -hairpin formation and rho 
protein 
post-transcriptional modification - Correct Answer️️ -the modifications that occur to an 
mRNA transcript before it is exported from the nucleus as mature mRNA. Include: 
splicing of introns, addit...
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