Streak plate techniques - Study guides, Class notes & Summaries
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BIOS 242 Week 1 Assignment; Lab 2 of 14 Onsite - Isolation of Pure Cultures
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BIOS 242 Week 1 Assignment; Lab 2 of 14 Onsite - Isolation of Pure Cultures 
 
Lab 2: Techniques for Isolation of Pure Cultures 
 
 
Learning Objectives: 
 
 
Isolate pure cultures from a mixed population of microorganisms using aseptic technique. 
Learn the streak plate method 
 
 
 
 
Introduction: 
 
 
Microorganisms exist in nature as mixed populations of different species. In order to study them and observe their characteristics it is first necessary to obtain pure cultures (a culture ...
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BIOS 242 Lab 1: Culture Transfer Techniques
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Lab 2: Techniques for Isolation of Pure Cultures 
Learning Objectives: 
 Isolate pure cultures from a mixed population of microorganisms using aseptic technique. 
 Learn the streak plate method 
VIDEO: 
 Making a streak Plate (Bio-Rad Laboratories) 

Introduction: 
Microorganisms exist in nature as mixed populations of different species. In order to study them and 
observe their characteristics it is first necessary to obtain pure cultures (a culture containing only one 
species of an o...
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Biol 1020 lab final Exam and A+ Answers
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Biol 1020 lab final Exam and A+ 
Answers 
Liquid media used to increase the number of microorganisms. - Broth 
Solid media used to isolate microbes - agar plate 
Normal flora bacteria used for studies involving osmotic pressure, antibiotic resistance, 
and biofilms. - S. epidermidis 
Clump of genetically identical microorganisms. - colony forming unit 
Single-celled fungi used for studies involving fermentation, genetics, and aging. - S. 
cerevisiae 
instrument used for spreading microorganis...
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FOOD MICROBIOLOGY FOR ETHIOPIAN HEALTH AND NUTRITION RESEARCH INSTITUTE (FOOD MICROBIOLOGY LABORATORY)
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LABORATORY MANUAL 
OF 
 FOOD MICROBIOLOGY 
FOR 
ETHIOPIAN HEALTH AND NUTRITION 
RESEARCH INSTITUTE 
(FOOD MICROBIOLOGY LABORATORY) 
UNIDO PROJECT 
(YA/ETH/03/436/11-52) 
DEC. 2003 
DRAFTED BY 
DR. CIIRA KIIYUKIA 
(INIDO / FOOD ANALYSIS – MICROBIOLOGY) 
1 
TABLE OF CONTENTS 
INTRODUCTION.....................................................................................................4 
MICROORGANISMS MORPHOLOGY AND STAINING.............7 
MICROSCOPY............................................
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Micro 2460 UTA AOBSN Lab Practical 1 (Labs 1, 2, 3, 4) Questions And Answers Rated A+ New Update Assured Satisfaction
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Ocular/eyepiece - Answer-2 eyepieces 
10x magnification 
move in or out to adjust 
positioned correct=single circle of light 
Body - Answer-Holds mirrors that bend light into oculars 
images reversed and inverted 
Nose piece - Answer-Where objectives are attached 
Objectives - Answer-Contain magnifying lenses 
4x, 10x, 40x, 100x 
Arm - Answer-Supports nose piece 
Holds power switch 
Holds focus adjustment knobs 
Stage - Answer-Flat platform that holds mechanical stage 
Mechanical Stage - Answer-...
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LABORATORY MANUAL OF FOOD MICROBIOLOGY FOR ETHIOPIAN HEALTH AND NUTRITION RESEARCH INSTITUTE (FOOD MICROBIOLOGY LABORATORY)
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MICROORGANISMS MORPHOLOGY AND STAINING.............7 
MICROSCOPY.....................................................................................................................7 
STAINED PREPARATIONS ...................................................................................................7 
MAKING A SMEAR.............................................................................................................8 
A SIMPLE STAIN........................................................
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Microbiology EXAM 1 (Chapters 1,3,4 and 6) Questions With Complete Answers.
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Microbiology EXAM 1 (Chapters 1,3,4 and 6) Questions With Complete Answers. 
 
microobiology 
The study of organisms too small to be seen with the naked eye is _______ 
viruses, bacteria, algae, protozoa, fungi and helminths 
What are the six major groups of microorganisms? 
3.5 billion years ago 
Single celled organisms arose ______ million years ago and have been shaping our earth ever since 
Eukaryotes = have a true nucleus (include protozoa, fungi, plants and animals) 
Prokaryotes= lack a tr...
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Microbiology Lab Review Questions with Certified Answers
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Microbiology Lab Review Questions with Certified Answers 
Explain why the body tube of the microscope should not be lowered while you are looking through the ocular lens. to ensure that the slide or lens is not broken 
For what purpose would you adjust the Iris diaphragm? adjust the amount of light coming through the specimen 
For what purpose would you adjust the course-adjustment knob? to bring the specimen into view 
For what purpose would you adjust the fine adjustment knob? to bring the spe...
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Microbiology LAB Exam #1 – Questions & Answers
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Convert Celsius to Fahrenheit - ANSWER - F = (9xC)/5 +32 
 
= How many decimel places, to the right of base - ANSWER - Six places --- .000 000 
 
nm= How many decimel places, to the right of base - ANSWER - Nine places --- . 
 
6.02x 10 = moving the decimel over how many spaces? - ANSWER - 23 
Compare and contrast these three techniques by where bacteria grow in the media - ANSWER - Streak Plate - bacteria grow only on the surface 
 Pour Plate - bacteria grow throughout the media 
 Spread Plate ...
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Microbiology Lab Exam 1 Questions and Answers Rated A
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Microbiology Lab Exam 1 Questions and Answers Rated A when viewing a smear with the oil immersion lens the final magnification is 1000x;10x ocular x 100X objective 
what is the purpose of heat fixing kill the bacteria, adhere to glass anddenature the proteins of bacteria 
name oneof the stains or regents used in the gram stain procedure gram crystal violet, gram iodine, gram sfaranin, 95% ethanol 
why are aseptic techniques used in the lab? to ensure that we do not contaminate what we are workin...
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