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L10 - Protein Synthesis II
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Second part of a two-part lecture on protein synthesis by Prof Robert Hindges
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L10 – Protein Synthesis II: Translation and AntibioticsSlide 3 - The central dogma of molecular biology
Slide 4-5 - Coding properties of nucleic acids
• Three key sets of experiments defined relationship between nuclear DNA and protein
expression
- A messenger exists that transmits information from the nucleus to the cytoplasm
(Brenner, 1960)
- The genetic code is read in triplets (Crick 1961, Nirenberg 1961, Khorana 1960s)
- The amino acid sequence is linearly related to the DNA sequence (Yanofsky, 1967)
Slide 6-9 – The genetic code
• The amino acid sequence of a protein is determined by the sequence of bases on the
DNA strand
• Pairs of bases not enough (only 16 possibilities)
• Triplets of bases more than enough (degenerate code)
• Phenylalanine is the only amino acid incorporated into protein using a cell-free system
, • Genetic code read in triplets
• Code degenerate
- 64 codons for 20 amino acids
- Three special termination codons
- All proteins start with the initiation codon AUG (methionine)
Slide 13-16 – Genetic code: reading frames
Slide 16-18 – Small changes can lead to severe consequences
• Point mutations
- Nonsense mutations: code for a stop, which can truncate the protein
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