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CHEM 120 Unit 7 Lab: OL Lab 13: Protein Synthesis (GRADED A)
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  • CHEM 120
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  • Chamberlain College Of Nursing

OL Lab 13: Protein Synthesis Introduction to General Chem 120 Learning Objectives • Explain the translation process from mRNA to amino acid • Explain post-translational modification of proteins • Explain protein synthesis processing in the ribosome • Explain the primary, secondary, tertiary...

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  • 2 de mayo de 2022
  • 5
  • 2021/2022
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  • chem 120 unit 7 lab ol lab 13 protein synthesis graded a

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  • Chamberlain College Of Nursing
  • CHEM 120
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CHEM120 OL, Week 7 Lab




OL Lab 13: Protein Synthesis



Introduction to General Chem 120




This study source was downloaded by 100000845689434 from CourseHero.com on 05 -02-2022 06:26:34 GMT -05:00


https://www.coursehero.com/file/65746278/OL-Lab-13-Protein-Synthesisdocx/

, CHEM120 OL, Week 7 Lab


Learning Objectives
• Explain the translation process from mRNA to amino acid
• Explain post-translational modification of proteins
• Explain protein synthesis processing in the ribosome
• Explain the primary, secondary, tertiary and quaternary structures of protein
• Explain the basic principles of mass spectrometry (MALDI-TOF)
Introduction
In the Protein Synthesis lab, you will learn about the difference between protein synthesis in
prokaryote (using E. coli) and eukaryote (using Chinese hamster ovarian (CHO) cells).
Prepare recombinant Erythropoietin and use the mass spectrometer
Your first task in the lab will be to prepare recombinant erythropoietin (EPO) that is transfected
into E. coli and CHO cells. The lab assistant will prepare the recombinant EPO and you will
measure the mass to charge ratio using a mass spectrometer. Not sure how to handle the mass
spectrometer? No worries! You can just take out your labpad and find an animated video to learn
the basics.
Study the translation process from mRNA to amino acids
You will also learn about the translation process from mRNA to amino acids and how amino
acids are assembled to proteins. A 3D animation will describe how codons are translated into
amino acids, how these amino acids are joined together by peptide bonds creating a primary
structure of protein, and how the primary structure is folded into secondary, tertiary and
quaternary structure.
Investigate doping in bike athletes
In the last part of the Protein Synthesis lab, you will use mass spectrometry to investigate if any
athletes are using recombinant human erythropoietin (rhEPO) as a doping substance. You will do
so by collaborating with the doping agent who collects urine samples at a large bicycle race.
Will you be able to detect if any of the athletes are using doping?


Part 1: Complete Labster Lab: Protein Synthesis
Part 2: Report and Reflection
Purpose: Describe in complete sentences and in your own words, the purpose of this experiment.
With this lab we are going to be learning the basics of protein synthesis and having to help
patients with chronic kidney disease. We will also be using a drug screening to catch someone
who is using a drug.




Observations: Record three observations from the simulation.



This study source was downloaded by 100000845689434 from CourseHero.com on 05 -02-2022 06:26:34 GMT -05:00


https://www.coursehero.com/file/65746278/OL-Lab-13-Protein-Synthesisdocx/

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