Cambridge International AS and A Level Biology Coursebook with CD-ROM
This is the whole chapter in a pdf. Most students have a hard time studying off the text book using the syllabus. This pdf just combines them both to make your life easier.
1.1 The microscope in cell studies .......................................3
Making temporary slides .............................................3
Differences between animal and plant cells ...................4
Units of measurement ...................................................6
Cell biology and microscopy ........................................7
Electron microscopy .....................................................9
1.2 Cells as the basic units of living organisms ...................13
Ultrastructure of an animal cell ...................................13
Ultrastructure of a plant cell ........................................14
Two fundamentally different types of cell .....................23
, 1 CELL STRUCTUR
1.1 T H E M I C R O S C O P E I N
STUDIES
A. compare the structure of typical animal and plant cells by making tempo
preparations of living material and using photomicrographs
Making temporary slides
1. Background information
- Biological material may be examined live or in preserved state; prepared sl
material that has been killed and preserved in life-like condition. This mat
into thin sections to enable light to pass through the structures for viewin
microscope; the sections are typically stained and ‘mounted’ on a glass slid
permanent preparation.
- Temporary preparations of fresh material have advantage that they can be
and useful for quick preliminary investigations; sectioning and staining m
carried out if required. Sometimes macerated (chopped up) material can b
examining structure of wood (xylem); a number of temporary stains are co
For example, iodine in potassium iodide solution useful for plant specime
starch blue-black and will also color nuclei and cell walls pale yellow. Dilu
methylene blue can be used to stain animal cells such as cheek cells.
- Viewing specimens yourself with microscope will help you understand and
structure more fully; this can be reinforced by making pencil drawing on g
plain paper. Remember always to draw what you see, and not what you th
see.
2. Procedure
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